Cui Jipeng, Yang Qianhui, Zhang Jiyue, Ju Chuanli, Cui Suxia
College of Life Sciences, Capital Normal University, Haidian District, Beijing 100048, China.
Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing 100048, China.
Int J Mol Sci. 2025 Jan 10;26(2):546. doi: 10.3390/ijms26020546.
As a globally distributed perennial Gramineae, can adapt to harsh ecological environments and has significant economic and environmental values. Here, we performed a complete assembly and annotation of the mitogenome of using genomic data from the PacBio and BGI platforms. The mitogenome is a multibranched structure of 501,134 bp, divided into two circular chromosomes of 325,493 bp and 175,641 bp, respectively. A sequence-simplified succinate dehydrogenase 4 gene was identified in this mitogenome, which is often translocated to the nuclear genome in the mitogenomes of gramineous species. We also identified tissue-specific mitochondrial differentially expressed genes using RNAseq data, providing new insights into understanding energy allocation and gene regulatory strategies in the long-term adaptive evolution of mitochondria. In addition, we studied the mitogenome features of in more detail, including repetitive sequences, gene Ka/Ks analyses, codon preferences, intracellular gene transfer, RNA editing, and multispecies phylogenetic analyses. Our results provide an essential molecular resource for understanding the genetic characterisation of the mitogenome of and provide a research basis for population genetics and species evolution in Arundiaceae.
作为一种全球分布的多年生禾本科植物，能适应恶劣的生态环境，具有重要的经济和环境价值。在此，我们利用来自PacBio和BGI平台的基因组数据，对该植物的线粒体基因组进行了完整的组装和注释。该植物线粒体基因组是一个501,134 bp的多分支结构，分别分为两条环状染色体，大小为325,493 bp和175,641 bp。在这个线粒体基因组中鉴定出一个序列简化的琥珀酸脱氢酶4基因，该基因在禾本科物种的线粒体基因组中常易位到核基因组中。我们还利用RNAseq数据鉴定了组织特异性线粒体差异表达基因，为理解该植物线粒体长期适应性进化中的能量分配和基因调控策略提供了新的见解。此外，我们更详细地研究了该植物的线粒体基因组特征，包括重复序列、基因Ka/Ks分析、密码子偏好、细胞内基因转移、RNA编辑和多物种系统发育分析。我们的结果为理解该植物线粒体基因组的遗传特征提供了重要的分子资源，并为芦竹科的群体遗传学和物种进化提供了研究基础。
