Lamula Siphamandla Q N, Taliwe Aphelele, Buwa-Komoreng Lisa V
Infectious Diseases and Medicinal Plants Research Niche Area, Botany Department, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
Int J Mol Sci. 2025 Jan 14;26(2):646. doi: 10.3390/ijms26020646.
(Thunb.) Less. has recently become a plant species of interest to researchers due to its biological activities and less toxic effects. Therefore, the aim of the study is to evaluate the in vitro anticancer potential and phytochemical constituents of plant extracts. Phytochemical screening and FTIR were carried out using standard methods. The antioxidant activity was accessed by determining its ability to scavenge the DPPH radical and nitric oxide radical, whereas the anticancer activity against prostate (DU-145 and PC-3), human T-lymphocyte (SKU-T), gastric adenocarcinoma (AGS), and human prostatic epithelial (PNTA1) cell line was evaluated using the MTT assay. The phytochemical analysis revealed the presence of tannins, flavonoids, saponins, steroids, terpenoids, and cardiac glycosides. The FTIR spectrum for the aqueous extract displayed characteristic peaks for O-H, C=O, C=C, and =C-H stretch. The aqueous ethanol and methanol extracts showed significant dose-dependent DPPH radical scavenging capacity. The aqueous, ethanol, and methanol extracts showed minimum NO scavenging activity of 4.3%, 9.6%, and 11.7% at 2500 µg/mL. The water extract demonstrated good activity against , , and with an MIC of 0.195 mg/mL. The ethanol and methanol extracts significantly reduced the percentage proliferation of DU-145, PC-3, and SKU-T cells at 100 μg/mL. These extracts demonstrated strong dose-dependent DPPH and NO scavenging and antibacterial and cell proliferation inhibition activities. The strong bioactivity of makes it a good candidate for the isolation and identification of active compounds for anticancer and related illnesses.
(Thumb.) Less. 由于其生物活性和较低的毒性作用，最近已成为研究人员感兴趣的植物物种。因此，本研究的目的是评估植物提取物的体外抗癌潜力和植物化学成分。采用标准方法进行植物化学筛选和傅里叶变换红外光谱（FTIR）分析。通过测定其清除二苯基苦味酰基自由基（DPPH）和一氧化氮自由基的能力来评估抗氧化活性，而使用MTT法评估其对前列腺（DU - 145和PC - 3）、人T淋巴细胞（SKU - T）、胃腺癌（AGS）和人前列腺上皮（PNTA1）细胞系的抗癌活性。植物化学分析表明存在单宁、黄酮类化合物、皂苷、甾体、萜类化合物和强心苷。水提取物的FTIR光谱显示了O - H、C = O、C = C和 = C - H伸缩振动的特征峰。乙醇和甲醇水提取物表现出显著的剂量依赖性DPPH自由基清除能力。在2500 μg/mL时，水、乙醇和甲醇提取物的最低NO清除活性分别为4.3%、9.6%和11.7%。水提取物对[此处原文缺失具体细胞名称]表现出良好的活性，最低抑菌浓度（MIC）为0.195 mg/mL。乙醇和甲醇提取物在100 μg/mL时显著降低了DU - 145、PC - 3和SKU - T细胞的增殖百分比。这些提取物表现出强剂量依赖性的DPPH和NO清除以及抗菌和细胞增殖抑制活性。[此处原文缺失具体植物名称]的强大生物活性使其成为分离和鉴定抗癌及相关疾病活性化合物的良好候选者。
