尼日利亚乙肝病毒和丁型肝炎病毒流行情况的分子流行病学、耐药变异及治疗意义：一项全国性研究

Molecular Epidemiology, Drug-Resistant Variants, and Therapeutic Implications of Hepatitis B Virus and Hepatitis D Virus Prevalence in Nigeria: A National Study.

作者信息

Adewuyi Oludare 'Sunbo, Balogun Muhammad Shakir, Otomaru Hirono, Abimiku Alash'le, Ahumibe Anthony Agbakizu, Ilori Elsie, Luong Que Anh, Mba Nwando, Avong James Christopher, Olaide John, Okunromade Oyeladun, Ahmad Adama, Akinpelu Afolabi, Ochu Chinwe Lucia, Olajumoke Babatunde, Abe Haruka, Ihekweazu Chikwe, Ifedayo Adetifa, Toizumi Michiko, Moriuchi Hiroyuki, Yanagihara Katsunori, Idris Jide, Yoshida Lay-Myint

机构信息

Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8523, Japan.

Department of Paediatric Infectious Diseases, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.

出版信息

Pathogens. 2025 Jan 20;14(1):101. doi: 10.3390/pathogens14010101.

DOI:10.3390/pathogens14010101

PMID:39861062

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11768191/

Abstract

Information on circulating HBV (sub-)genotype, variants, and hepatitis D virus (HDV) coinfection, which vary by geographical area, is crucial for the efficient control and management of HBV. We investigated the genomic characteristics of HBV (with a prevalence of 8.1%) and the prevalence of HDV in Nigeria. We utilised 777 HBV-positive samples and epidemiological data from the two-stage sampled population-based, nationally representative Nigeria HIV/AIDS Indicator and Impact Survey conducted in 2018. We assessed 732 HBV DNA-extracted samples with detectable viral loads (VLs) for (sub-)genotypes and variants by whole-genome pre-amplification, nested PCR of the -and -gene, and BigDye Terminator sequencing. We conducted HDV serology. In total, 19 out of the 36 + 1 states in Nigeria had a high prevalence of HBV (≥8%), with the highest prevalence (10.4%) in the north-central geopolitical zone. Up to 33.2% (95% CI 30.0-36.6) of the participants had detectable VLs of ≥300 copies/mL. The predominant circulating HBV genotype was E with 98.4% (95% CI 97.1-99.1), followed by A with 1.6% (95% CI 0.9-2.9). Drug-resistant associated variants and immune escape variants were detected in 9.3% and 0.4%, respectively. The seroprevalence of HDV was 7.34% (95% CI 5.5-9.2). Nigeria has subtype E as the major genotype with many variants.

摘要

有关循环HBV（亚）基因型、变异体以及丁型肝炎病毒（HDV）合并感染的信息因地理区域而异，对于高效控制和管理HBV至关重要。我们调查了尼日利亚HBV的基因组特征（患病率为8.1%）以及HDV的患病率。我们利用了777份HBV阳性样本以及2018年进行的两阶段基于人群抽样的、具有全国代表性的尼日利亚艾滋病毒/艾滋病指标和影响调查的流行病学数据。我们通过全基因组预扩增、 -和 -基因的巢式PCR以及BigDye终止子测序，对732份提取了HBV DNA且病毒载量（VL）可检测的样本进行了（亚）基因型和变异体评估。我们进行了HDV血清学检测。尼日利亚36 + 1个州中共有19个州HBV患病率较高（≥8%），其中最高患病率（10.4%）出现在中北部地缘政治区。高达33.2%（95%置信区间30.0 - 36.6）的参与者病毒载量可检测到≥300拷贝/毫升。主要流行的HBV基因型是E型，占98.4%（95%置信区间97.1 - 99.1），其次是A型，占1.6%（95%置信区间0.9 - 2.9）。分别在9.3%和0.4%的样本中检测到耐药相关变异体和免疫逃逸变异体。HDV血清阳性率为7.34%（95%置信区间5.5 - 9.2）。尼日利亚以E亚型作为主要基因型且存在许多变异体。