Eisa Eman Abdelhakim, Pasquel Davila Daniela Salome, Ördögh Máté
Department of Floriculture and Dendrology, Institute of Landscape Architecture, Urban Planning and Garden Art, Hungarian University of Agriculture and Life Sciences (MATE), Villányi Street 29-43, 1118 Budapest, Hungary.
Botanical Gardens Research Department, Horticulture Research Institute, Agricultural Research Center (ARC), Giza 12619, Egypt.
Plants (Basel). 2025 Jan 9;14(2):172. doi: 10.3390/plants14020172.
This study investigates the acclimatization success of 'Fire', a popular ornamental bromeliad, through in vitro propagation on various substrates. Due to the increasing demand for , micropropagation offers a promising solution to overcome the limitations of traditional propagation methods. In this research, acclimatization was conducted in two trial types: in the one-step greenhouse conditions, and in two-step acclimatization, which introduced a controlled laboratory step before transferring plants to the greenhouse. The substrates examined included pure and mixed forms of turf, perlite, coco coir, pine bark (hereafter referred to as bark), moss, and vermiculite. Morphological traits such as plant height, leaf length, number and length of roots, and fresh weight were evaluated, together with physiological parameters, such as chlorophyll and carotenoid concentrations and survival percentage, to test the effectiveness of acclimatization. Coco coir-based substrates significantly enhanced plant height, root development, and survival percentages in both experiments compared with other substrates, thus proving its suitability for the propagation of . Vermiculite had the highest survival rate during one-step acclimatization, whereas turf showed a very good performance in two-step acclimatization. On the opposite side, substrates containing bark and moss showed a reduced effect on plant growth and survival, which indicated the vital role of substrates for best development. Statistical analyses confirmed the superiority of some combinations of substrates related to physiological health, showing that optimal acclimatization results could be improved by a chosen substrate. These results strengthen the present in vitro propagation protocols of the species by confirming the relevance of substrate choice in producing hardy plants with good commercial prospects.
本研究通过在各种基质上进行离体繁殖，调查了一种流行的观赏凤梨科植物“火焰”的驯化成功率。由于对[具体需求内容缺失]的需求不断增加，微繁殖为克服传统繁殖方法的局限性提供了一个有前景的解决方案。在本研究中，驯化在两种试验类型中进行：一步温室条件下，以及两步驯化，即在将植物转移到温室之前引入一个受控的实验室步骤。所检查的基质包括草皮、珍珠岩、椰壳纤维、松树皮（以下简称树皮）、苔藓和蛭石的纯形式和混合形式。评估了植物高度、叶片长度、根的数量和长度以及鲜重等形态特征，以及叶绿素和类胡萝卜素浓度和存活率等生理参数，以测试驯化的有效性。与其他基质相比，基于椰壳纤维的基质在两个实验中均显著提高了植物高度、根系发育和存活率，从而证明了其对[具体植物名称缺失]繁殖的适用性。蛭石在一步驯化过程中存活率最高，而草皮在两步驯化中表现良好。相反，含有树皮和苔藓的基质对植物生长和存活的影响较小，这表明基质对最佳发育起着至关重要的作用。统计分析证实了某些基质组合在生理健康方面的优越性，表明选择合适的基质可以改善最佳驯化结果。这些结果通过确认基质选择在培育具有良好商业前景的耐寒植物方面的相关性，加强了目前该[具体植物名称缺失]物种的离体繁殖方案。
