Zengi N Rukiye, Erdoğan Selim, Özhan Onural, Karaca Elif Taşlidere, Özçinar Semih, Yilmazteki N Yakup, Yağin Fatma Hilal, Uğur Yılmaz, Firat Cemal, Parlakpinar Hakan, Uyumlu Ayşe Burçin
Republic of Türkiye Ministry of Agriculture and Forestry, General Directorate of Agricultural Research and Policies, Apricot Research Institute, Malatya, Türkiye.
Pharmacy Faculty, Inonu University, Malatya, Türkiye.
Burns. 2025 Mar;51(2):107391. doi: 10.1016/j.burns.2025.107391. Epub 2025 Jan 14.
In the burn affected area of the skin, the progression or deepening of wounds is related to oxidative stress. Especially in the highly susceptible stasis zone, tissues survive to the extent that they can cope with oxidative stress.
This study investigated the potential of extracts (E) derived from the fruits (F) and leaves (L) of elderberry (E), chokeberry (C), and black mulberry (M), which are rich in antioxidant properties, to enhance the recovery of the stasis zone in burn wounds.
The study employed a sample size of 56 healthy rats. The comb burn model was used. The rats were administered the extracts via gastric gavage for a period of 21 days. Histological examination and biochemical analyses were conducted on biopsies taken from the stasis zone on the 3rd, 7th, and 21st days of the study. Photography was performed for macroscopic evaluations. The histological assays included the evaluation of inflammatory cell infiltration, reepithelialization, and collagenization, as well as immunohistochemical analysis of vascular endothelial growth factor (VEGF). Bioassays pertaining to the enzymatic activities of catalase, superoxide dismutase, glutathione peroxidase, and malondialdehyde (MDA) levels were performed.
In macroscopic evaluation, a significant difference was found between the groups in terms of stasis area (F=3.58, p< 0.001). Post-hoc analyses showed that there was a significant difference between CFE-ELE, EFE-Burn, ELE-Burn and MLE-Burn groups in terms of stasis zones (p < 0.05). However, the difference between the groups according to time was not significant (F=1.36, p = 0.16). At the end of the 21-day experiment, inflammatory cell infiltration was higher in the burn group compared to the other groups, but similar to the CFE group. Re-epithelialization was similar in the burn group compared to the fruit extract groups and significantly lower compared to the leaf extract groups. Furthermore, a significant increase in collagenization and VEGF immunoreactivity was observed in all treatment groups compared to the burn group (p < 0.05). However, no significant difference was detected between the treatment groups. The treatment groups presented a notable reduction in MDA levels in comparison to the burn group (p < 0.001).
This study demonstrated the efficacy of fruit and leaf extracts in burn healing. Histological examination revealed that leaf extracts exhibited superior healing effects compared to fruit extracts. These results suggest that bioactive components in fruits and leaves may have different biological effects.
在皮肤烧伤区域，伤口的进展或加深与氧化应激有关。特别是在高度易感的淤滞区，组织的存活程度取决于它们应对氧化应激的能力。
本研究调查了富含抗氧化特性的接骨木果（E）、黑果腺肋花楸果（C）和黑桑果（M）及其叶提取物（E）促进烧伤创面淤滞区恢复的潜力。
本研究采用了56只健康大鼠作为样本。使用梳状烧伤模型。通过胃管给大鼠灌胃提取物，持续21天。在研究的第3天、第7天和第21天，对从淤滞区采集的活检组织进行组织学检查和生化分析。进行摄影以进行宏观评估。组织学检测包括评估炎症细胞浸润、再上皮化和胶原化，以及血管内皮生长因子（VEGF）的免疫组织化学分析。进行了与过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶的酶活性和丙二醛（MDA）水平相关的生物测定。
在宏观评估中，各实验组在淤滞面积方面存在显著差异（F=3.58，p<0.001）。事后分析表明，CFE-ELE、EFE-Burn、ELE-Burn和MLE-Burn组在淤滞区方面存在显著差异（p<0.05）。然而，各实验组之间随时间的差异不显著（F=1.36，p=0.16）。在21天实验结束时，烧伤组的炎症细胞浸润高于其他组，但与CFE组相似。烧伤组的再上皮化与果实提取物组相似，但与叶提取物组相比显著降低。此外，与烧伤组相比，所有治疗组的胶原化和VEGF免疫反应性均显著增加（p<0.05）。然而，各治疗组之间未检测到显著差异。与烧伤组相比，治疗组的MDA水平显著降低（p<0.001）。
本研究证明了果实和叶提取物在烧伤愈合中的有效性。组织学检查显示，叶提取物比果实提取物表现出更好的愈合效果。这些结果表明，果实和叶中的生物活性成分可能具有不同的生物学效应。
