Efficacy of Formulation With Potential as Herbal Medicine on Second Degree Burn Wound: Biochemical and Molecular Evaluation.

BACKGROUND

Burn injury is a condition caused by heat, cold, electricity, synthetic substances, and radiation, and it causes psychological and physical problems in the affected individuals.

AIMS

In this study, it was aimed to investigate the healing effect of the spray formulation prepared using ethanol extracts of Olea europaea and Aloe vera leaves, Cocus nucifera fruit, and Chamomilla recutita flower plants (OACC) in a second-degree burn model created in rats, using biochemical and molecular parameters.

METHODS

Experimental groups were assigned to Healthy control (HC), Burn control (BC), Silver-Sulfadiazine (SS) and OACC. A deep second-degree burn was induced on the lower back and upper back of each rat under standard burning procedures, respectively. Experiments were performed using serum and skin tissue samples obtained on the 3rd-21st days after the burns were created. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were calculated. Transforming Growth Factor Beta-1 (Tgf-β1), Vascular Endothelial Growth Factor-alfa (Vegf-α), interleukin-6 (Il-6) and Tumor Necrosis Factor Alpha (Tnf-α) mRNA expression levels were determined using real-time polymerase chain reaction (RT-PCR).

RESULTS

AOCC reduced the increased MDA levels in serum related to the burning event, while increasing the decreased SOD enzyme activity levels. In addition, AOCC decreased the gene expression levels of Tgf-β1 and Vegf-α, which are growth factors that were increased in the burn group, and Il-6 and Tnf-α, which are oxidative stress markers.

CONCLUSIONS

We believe that our study will shed light on the detailed examination of biochemical and molecular pathways affecting the wound healing process in future studies and will contribute to opening new doors for treatment.