Askin Seda, Kaynarpinar Merve
Health Services Vocational School, Ataturk University, Erzurum, Turkey.
Institute of Health Sciences, Ataturk University, Erzurum, Turkey.
J Cosmet Dermatol. 2025 Mar;24(3):e70122. doi: 10.1111/jocd.70122.
Burn injury is a condition caused by heat, cold, electricity, synthetic substances, and radiation, and it causes psychological and physical problems in the affected individuals.
In this study, it was aimed to investigate the healing effect of the spray formulation prepared using ethanol extracts of Olea europaea and Aloe vera leaves, Cocus nucifera fruit, and Chamomilla recutita flower plants (OACC) in a second-degree burn model created in rats, using biochemical and molecular parameters.
Experimental groups were assigned to Healthy control (HC), Burn control (BC), Silver-Sulfadiazine (SS) and OACC. A deep second-degree burn was induced on the lower back and upper back of each rat under standard burning procedures, respectively. Experiments were performed using serum and skin tissue samples obtained on the 3rd-21st days after the burns were created. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were calculated. Transforming Growth Factor Beta-1 (Tgf-β1), Vascular Endothelial Growth Factor-alfa (Vegf-α), interleukin-6 (Il-6) and Tumor Necrosis Factor Alpha (Tnf-α) mRNA expression levels were determined using real-time polymerase chain reaction (RT-PCR).
AOCC reduced the increased MDA levels in serum related to the burning event, while increasing the decreased SOD enzyme activity levels. In addition, AOCC decreased the gene expression levels of Tgf-β1 and Vegf-α, which are growth factors that were increased in the burn group, and Il-6 and Tnf-α, which are oxidative stress markers.
We believe that our study will shed light on the detailed examination of biochemical and molecular pathways affecting the wound healing process in future studies and will contribute to opening new doors for treatment.
烧伤是由热、冷、电、合成物质及辐射引起的一种病症，会给受影响个体带来心理和身体问题。
本研究旨在利用生化和分子参数，在大鼠建立的二度烧伤模型中，研究使用油橄榄叶、库拉索芦荟叶、椰子果和洋甘菊花植物（OACC）的乙醇提取物制备的喷雾制剂的愈合效果。
将实验组分为健康对照组（HC）、烧伤对照组（BC）、磺胺嘧啶银组（SS）和OACC组。在标准烧伤程序下，分别在每只大鼠的下背部和上背部诱导深二度烧伤。使用烧伤后第3天至21天获得的血清和皮肤组织样本进行实验。计算丙二醛（MDA）和超氧化物歧化酶（SOD）水平。使用实时聚合酶链反应（RT-PCR）测定转化生长因子β-1（Tgf-β1）、血管内皮生长因子α（Vegf-α）、白细胞介素-6（Il-6）和肿瘤坏死因子α（Tnf-α）的mRNA表达水平。
AOCC降低了与烧伤事件相关的血清中升高的MDA水平，同时提高了降低的SOD酶活性水平。此外，AOCC降低了烧伤组中升高的生长因子Tgf-β1和Vegf-α以及氧化应激标志物Il-6和Tnf-α的基因表达水平。
我们相信，我们的研究将为未来研究中影响伤口愈合过程的生化和分子途径的详细研究提供线索，并为开辟新的治疗途径做出贡献。
