Kaewtong Chatthai, Norrasarn Jukkraphop, Wanno Banchob, Hayami Shinya, Tuntulani Thawatchai, Pulpoka Buncha
Nanotechnology Research Unit and Supramolecular Chemistry Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha Sarakham, 44150, Thailand.
Department of Chemistry, Graduate School of Science and Technology, Institute of Industrial Nanomaterials (IINa), Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto, 860-8555, Japan.
Chem Asian J. 2025 May 2;20(9):e202401596. doi: 10.1002/asia.202401596. Epub 2025 Feb 8.
This research focuses on the selective detection of Hg ions using hybrid nanosensors composed of rhodamine building blocks linked to polyamine units of varying chain lengths to produce Rho1-Rho4, which were subsequently conjugated with thioctic acid (RT1-RT4) and attached to the surface of gold nanoparticles to create hybrid nanosensors (GRT1-GRT4) designed for detecting heavy metals. The chemical structures, purity, morphology, and chemical composition were characterized through XRD, NMR, TEM, ATR-FTIR, and mass spectrometry. These hybrid nanosensors demonstrated excellent selectivity and sensitivity in colorimetric and fluorescence responses towards Hg, outperforming other metal ions. In their spirolactam form, the sensors were non-fluorescent but transformed into a fluorescent form upon interaction with Hg, resulting in enhanced fluorescence and colorimetric changes. Theoretical calculations indicated that Hg could form stable complexes with the RT1 sensor by binding to the oxygen and nitrogen atoms in the spirolactam structure, as well as coordinating with the oxygen atom of the amide bond. The detection limit (LOD) was 1.68×10 M with a response time of less than 40 seconds. This method offers a simple and highly sensitive approach for detecting Hg ions in both environmental and biological applications, as confirmed by the characterization and experimental data.
本研究聚焦于使用由罗丹明结构单元与不同链长的多胺单元相连以生成Rho1 - Rho4的混合纳米传感器对汞离子进行选择性检测，随后将其与硫辛酸共轭（RT1 - RT4）并附着于金纳米颗粒表面，以创建用于检测重金属的混合纳米传感器（GRT1 - GRT4）。通过X射线衍射（XRD）、核磁共振（NMR）、透射电子显微镜（TEM）、衰减全反射傅里叶变换红外光谱（ATR - FTIR）和质谱对其化学结构、纯度、形态和化学成分进行了表征。这些混合纳米传感器在对汞的比色和荧光响应中表现出优异的选择性和灵敏度，优于其他金属离子。在其螺内酰胺形式下，传感器无荧光，但与汞相互作用时会转变为荧光形式，导致荧光增强和比色变化。理论计算表明，汞可通过与螺内酰胺结构中的氧和氮原子结合以及与酰胺键的氧原子配位，与RT1传感器形成稳定的络合物。检测限（LOD）为1.68×10 M，响应时间小于40秒。表征和实验数据证实，该方法为环境和生物应用中检测汞离子提供了一种简单且高度灵敏的方法。
