Choudhary Bhavesh, Pawase Anil S, Ghode Gajanan S, Tibile Raju M, Bhatkar Varsha R, Choudhary Divyashree, Choudhary Utkarsh
College of Fisheries (Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli), Shirgaon, Ratnagiri, 415629, Maharashtra, India.
College of Fisheries, Central Agricultural University (Imphal), Lembucherra, Agartala, 799210, Tripura, India.
Heliyon. 2025 Jan 5;11(1):e41712. doi: 10.1016/j.heliyon.2025.e41712. eCollection 2025 Jan 15.
Intensification of shrimp farming practices has increased the number and severity of disease outbreaks globally. As a result, diseases have become a significant barrier to profitable and sustainable shrimp production. Shrimp farming practices are reviving in India after its downfall in the late 90s. However, these farming practices also witness disease outbreaks due to viral and bacterial infections. Among the bacterial infections, Vibrios are the most important bacterial causative agents found in shrimp farms. They are ubiquitous and invariably seen in shrimp production conditions as opportunistic pathogens. The present study was conducted to identify the bacterial pathogens associated with the shrimp farming systems along the Ratnagiri coast. In all, two farming units were selected: Varavade farm - a six-year-old farm, and Chinchkhari farm, a new virgin farm. The water and sediment samples were collected from January to May 2022 throughout culture period of one crop. The total plate count (TPC) of the shrimp farm water samples of the Varavade farm varied from 4.35 to 6.32 log10 CFU mL. In the sediments, the minimum value of TPC was 4.99 log10 CFU g, while the maximum value observed was 7.25 log10 CFU g. The Total count (TVC) of water samples from Varavade farm varied from 4.01 to 5.63 log10 CFU mL. In the sediments, the minimum value of TVC was 4.64, while the maximum value observed was 6.56 log10 CFU g. The statistical analysis showed a significant difference in TPC and TVC ( < 0.05) among different days of culture. The TPC of the shrimp farm water samples of the Chinchkhari farm varied from 5.22 to 8.17 log10 CFU mL. In the sediment, the minimum value of TPC was 5.87, while the maximum value was observed at 8.45 log10 CFU g. The TVC of water samples from the Chinchkhari farm varied from 4.75 to 6.89 log10 CFU mL. In the sediment, the minimum value of TVC was 5.16, while the maximum value observed was 6.70 log10 CFU g. The statistical analysis showed a significant difference in TPC and TVC ( < 0.05) among different days of culture. The bacterial load was observed to increase with the progression of the culture period on both farms. The usage of probiotics, chemicals, and water exchange was observed to promote a decrease in the bacterial community.
对虾养殖方式的集约化增加了全球疾病暴发的数量和严重程度。因此，疾病已成为对虾盈利性和可持续生产的重大障碍。印度的对虾养殖在20世纪90年代后期衰落之后正在复苏。然而，这些养殖方式也因病毒和细菌感染而出现疾病暴发。在细菌感染中，弧菌是对虾养殖场中发现的最重要的细菌病原体。它们无处不在，在对虾生产环境中总是作为机会性病原体出现。本研究旨在确定与拉特纳吉里海岸对虾养殖系统相关的细菌病原体。总共选择了两个养殖单元：Varavade养殖场——一个有六年历史的养殖场，以及Chinchkhari养殖场，一个新的未开发养殖场。在2022年1月至5月整个一季养殖期间采集水和沉积物样本。Varavade养殖场对虾养殖池水样本的总平板计数（TPC）在4.35至6.32 log10 CFU/mL之间变化。在沉积物中，TPC的最小值为4.99 log10 CFU/g，而观察到的最大值为7.25 log10 CFU/g。Varavade养殖场水样本的总菌数（TVC）在4.01至5.63 log10 CFU/mL之间变化。在沉积物中，TVC的最小值为4.64，而观察到的最大值为6.56 log10 CFU/g。统计分析表明，不同养殖天数的TPC和TVC存在显著差异（<0.05）。Chinchkhari养殖场对虾养殖池水样本的TPC在5.22至8.17 log10 CFU/mL之间变化。在沉积物中，TPC的最小值为5.87，而观察到的最大值为8.45 log10 CFU/g。Chinchkhari养殖场水样本的TVC在4.75至6.89 log10 CFU/mL之间变化。在沉积物中，TVC的最小值为5.16，而观察到的最大值为6.70 log10 CFU/g。统计分析表明，不同养殖天数的TPC和TVC存在显著差异（<0.05）。在两个养殖场都观察到细菌负荷随着养殖期的推进而增加。观察到使用益生菌、化学品和换水可促使细菌群落减少。
