使用表面增强拉曼光谱（SERS）对具有不同耐药性的[具体对象]进行单细胞分析。（原文中“of ”后面缺少具体内容）

Zyubin Andrey, Lavrova Anastasia, Dogonadze Marine, Borisov Evgenii, Postnikov Eugene B

Immanuel Kant Baltic Federal University, Kaliningrad, Russia.

Saint-Petersburg State Research Institute of Phthisiopulmonology, Saint-Petersburg, Russia.

PeerJ. 2025 Jan 24;13:e18830. doi: 10.7717/peerj.18830. eCollection 2025.

In this work, we investigated individual bacteria belonging to strains of the Beijing family with different drug sensitivity (sensitive, multi and extensive drug-resistant) by surface-enhanced Raman spectroscopy (SERS) in the fingerprint region. The latter is focused on the spectral bands, which correspond to a set of glutathione bands and DNA methylation patterns revealed due to 5-methylcytosine spectral biomarkers. It is shown that these spectral features can be correlated with drug sensitivity and DNA methylation. Thus, since this kind of diagnostics is fast and operates with individual cells, it can be considered a promising tool, which significantly shortens the time required for a strain's type identification necessary to prescribe adequate therapy.

在这项工作中，我们通过表面增强拉曼光谱（SERS）在指纹区域研究了属于北京家族不同药敏性（敏感、多重耐药和广泛耐药）菌株的单个细菌。后者聚焦于光谱带，这些光谱带对应于一组谷胱甘肽带以及由于5-甲基胞嘧啶光谱生物标志物而揭示的DNA甲基化模式。结果表明，这些光谱特征可以与药敏性和DNA甲基化相关联。因此，由于这种诊断方法快速且针对单个细胞进行操作，它可以被视为一种有前景的工具，能够显著缩短确定适当治疗所需菌株类型鉴定所需的时间。