Thomas L L, Sturk A, Kahlé L H, ten Cate J W
Clin Chim Acta. 1981 Oct 8;116(1):63-8. doi: 10.1016/0009-8981(81)90169-8.
A method is described for the quantitative determination of endotoxins in blood. The method is based upon the endotoxin-dependent activation of a proenzyme present in Limulus amebocyte lysate. This activated enzyme is measured by using the chromogenic substrate S 2422. Inhibitors and activated coagulation factors possibly interfering in the assay are removed by dilution and boiling. The method has been proven to be fast (2.5 h), sensitive and reproducible with a detection limit of 10 ng/l. Preliminary results comparing the results of blood cultures with the endotoxin assay indicate a good correlation.
本文描述了一种定量测定血液中内毒素的方法。该方法基于鲎试剂中存在的一种酶原的内毒素依赖性激活。通过使用显色底物S 2422来测量这种激活的酶。通过稀释和煮沸去除可能干扰测定的抑制剂和活化凝血因子。该方法已被证明快速（2.5小时）、灵敏且可重复，检测限为10 ng/l。将血培养结果与内毒素测定结果进行比较的初步结果表明两者具有良好的相关性。
