Ibrahim Sara, Ismail Mohammed, Abdelrahman Taghrid, Sharkawy Mona, Abdellatif Ahmed, Allam Nageh K
Basic Medical Science Department, Faculty of Dentistry, Al-Ryada University for Science and Technology Menofia Egypt.
Zoology Department, Faculty of Science, Cairo University Giza Egypt.
Nanoscale Adv. 2025 Jan 9;7(6):1572-1581. doi: 10.1039/d4na00885e. eCollection 2025 Mar 11.
Oxidative stress plays a major role in the secondary injury of the spinal cord tissue due to the high lipid content of nervous tissue. In the present study, coaxial nanofibers were loaded with the natural antioxidant pyrroloquinoline quinone (PQQ) and used as an implantable drug-delivery system and a scaffold post-SCI. The obtained data show that the concentration of NO and the activity of inducible nitric oxide synthase (iNOS) were significantly ( < 0.05) increased in the spinal cord injury (SCI) group. These levels were significantly decreased following treatment with nanofibers/PQQ. Implantation of nanofibers/PQQ resulted in a significant ( < 0.05) drop in the level of malondialdehyde (MDA) compared to the SCI group. The application of nanofibers loaded with PQQ after SCI caused a significant ( < 0.05) elevation of superoxide dismutase (SOD) and catalase (CAT) activity in the spinal cord tissue. The present work shows the protective role of coaxial nanofibers loaded with PQQ against oxidative stress in spinal cord injury. The reversal of oxidative stress with PQQ can lead to better outcomes following spinal cord injury.
由于神经组织中脂质含量高，氧化应激在脊髓组织的继发性损伤中起主要作用。在本研究中，同轴纳米纤维负载天然抗氧化剂吡咯喹啉醌（PQQ），用作植入式药物递送系统和脊髓损伤后的支架。获得的数据表明，脊髓损伤（SCI）组中一氧化氮（NO）浓度和诱导型一氧化氮合酶（iNOS）活性显著（<0.05）升高。用纳米纤维/PQQ处理后，这些水平显著降低。与SCI组相比，植入纳米纤维/PQQ导致丙二醛（MDA）水平显著（<0.05）下降。脊髓损伤后应用负载PQQ的纳米纤维导致脊髓组织中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性显著（<0.05）升高。目前的工作表明负载PQQ的同轴纳米纤维对脊髓损伤中的氧化应激具有保护作用。用PQQ逆转氧化应激可导致脊髓损伤后获得更好的结果。
