Mahdizadeh Maryam, Mahdavi Nazanin, Bijani Ali, Bijani Fatima
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
BMC Oral Health. 2025 Jan 29;25(1):158. doi: 10.1186/s12903-025-05522-4.
Calretinin is a 29 kilodalton (KDa) calcium-binding protein that is expressed in normal and tumoral tissues. The expression of calretinin has been shown in the dental epithelium during odontogenesis and in different odontogenic cysts and tumors such as ameloblastoma. Since the epithelium of calcifying odontogenic cyst (COC) is similar to ameloblastoma and in both lesions, an arrangement of loose cells similar to stellate reticulum is seen, we aimed to investigate the comparative expression of calretinin in COC and ameloblastoma.
In this observational-analytical study, 11 paraffin blocks of each lesion were examined. The immunohistochemical expression of calretinin and its severity and distribution were evaluated. Data were evaluated using SPSS version 26 and P < 0.05 was considered the level of significance.
Out of 11 ameloblastoma samples, calretinin staining was positive in 6 samples in the stellate reticulum-like cells and also in the lining of microcysts and macrocysts in some cases. Ameloblast like cells did not show staining. In COC samples, epithelial cells were not stained, but in 8 samples, staining was seen in the area of ghost cells. There was no significant difference in staining between ameloblastoma and COC (P = 0.33), but the difference in staining in the stellate reticulum of ameloblastoma and COC was significant. (P = 0.012).
it is concluded that calretinin does not play a role in the pathogenesis and histogenesis of COC epithelium, but since ghost cells are caused by a degenerative process, the possibility of involving calretinin as an apoptotic factor in the formation of these cells is supported.
钙视网膜蛋白是一种29千道尔顿（kDa）的钙结合蛋白，在正常组织和肿瘤组织中均有表达。钙视网膜蛋白的表达已在牙胚发育过程中的牙上皮以及不同的牙源性囊肿和肿瘤（如成釉细胞瘤）中得到证实。由于钙化牙源性囊肿（COC）的上皮与成釉细胞瘤相似，并且在这两种病变中都可见到类似于星网状层的松散细胞排列，我们旨在研究钙视网膜蛋白在COC和成釉细胞瘤中的表达差异。
在这项观察性分析研究中，对每种病变的11个石蜡块进行了检查。评估了钙视网膜蛋白的免疫组化表达及其强度和分布。使用SPSS 26版对数据进行评估，P < 0.05被认为具有统计学意义。
在11个成釉细胞瘤样本中，6个样本的星网状层样细胞以及某些情况下微囊肿和大囊肿的内衬中钙视网膜蛋白染色呈阳性。成釉细胞样细胞未显示染色。在COC样本中，上皮细胞未染色，但在8个样本中，在影细胞区域可见染色。成釉细胞瘤和COC之间的染色无显著差异（P = 0.33），但成釉细胞瘤和COC的星网状层染色差异显著（P = 0.012）。
得出结论，钙视网膜蛋白在COC上皮的发病机制和组织发生中不起作用，但由于影细胞是由退行性过程引起的，因此支持钙视网膜蛋白作为凋亡因子参与这些细胞形成的可能性。
