俄罗斯西北部特定地区硬蜱中蜱传脑炎病毒的流行情况及遗传多样性

Prevalence and genetic diversity of tick-borne encephalitis virus in ixodid ticks from specific regions of northwestern Russia.

作者信息

Sharova Alena A, Tokarevich Nikolay K, Baimova Regina R, Freylikhman Olga A, Karmokov Islam A, Riabiko Ekaterina G, Lunina Gelena A, Buzinov Roman V, Sokolova Olga V, Buts Lidia V, Bespyatova Lubov A, Bubnova Liliya A, Safonova Olga S, Kalinina Elena L, Stankevich Andrey I, Vikse Rose, Andreassen Ashild K, Gladkikh Anna S, Forghani Majid, Gritseva Anastasia S, Popova Margarita R, Ramsay Edward S, Dedkov Vladimir G

机构信息

Saint Petersburg Pasteur Institute, St. Petersburg, Russia.

North-Western State Medical University named after I. I. Mechnikov, St. Petersburg, Russia.

出版信息

PLoS One. 2025 Jan 30;20(1):e0314385. doi: 10.1371/journal.pone.0314385. eCollection 2025.

DOI:10.1371/journal.pone.0314385

PMID:39883660

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11781730/

Abstract

Russia is a country with a high incidence of tick-borne encephalitis (TBE). In northwestern regions of Russia, 110 TBE cases were registered in 2021. The largest numbers of TBE cases were registered in the Arkhangelsk region and St. Petersburg. TBEV seropositivity among healthy individuals, including the unvaccinated population in northwestern Russia, was found in 12.2% of studied samples, indicating active TBEV circulation. The prevalence of TBEV is 2.4% in the two tick species most common in northwestern regions of Russia, Ixodes ricinus and Ixodes persulcatus. However, there is still no comprehensive data on the molecular characterization and phylogenetic analysis of the circulating TBEV strains. The purpose of the study was to determine the prevalence of TBEV and to identify its subtypes in ixodid ticks collected in specific areas of northwestern Russian regions. Phylogenetic analysis of E protein sequences of ten obtained strains showed that they all belong to the Siberian subtype, which were clustered into two groups: the most numerous Baltic group, clusteron 3D; and the Vasilchenko group. However, some unique isolates may form new clusterons.

摘要

俄罗斯是蜱传脑炎（TBE）高发国家。2021年，俄罗斯西北部地区登记了110例蜱传脑炎病例。蜱传脑炎病例数最多的是阿尔汉格尔斯克地区和圣彼得堡。在包括俄罗斯西北部未接种疫苗人群在内的健康个体中，12.2%的研究样本检测到蜱传脑炎病毒血清阳性，表明蜱传脑炎病毒正在活跃传播。在俄罗斯西北部最常见的两种蜱类，蓖麻硬蜱和全沟硬蜱中，蜱传脑炎病毒的流行率为2.4%。然而，目前仍缺乏关于蜱传脑炎病毒流行毒株分子特征和系统发育分析的全面数据。本研究的目的是确定蜱传脑炎病毒在俄罗斯西北部特定地区采集的硬蜱中的流行率，并鉴定其亚型。对获得的10个毒株的E蛋白序列进行系统发育分析表明，它们均属于西伯利亚亚型，分为两组：数量最多的波罗的海组，聚类在3D；以及瓦西里琴科组。然而，一些独特的分离株可能会形成新的聚类。