Moolsup Furoida, Sukketsiri Wanida, Sianglum Wipawadee, Saetan Jirawat, Khumpirapang Nattakanwadee, Tanasawet Supita
Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand.
Laboratory Animal Service Center, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand.
Adv Pharmacol Pharm Sci. 2025 Jan 22;2025:7198281. doi: 10.1155/adpp/7198281. eCollection 2025.
Recently, seaweed extracts have been found to have potential in skin benefits. This study, therefore, aimed to explore phytochemical analysis, antimicrobial, antioxidant, and wound healing properties of brown seaweed ethanolic extract (SPEE) on human skin keratinocyte HaCaT cells and the possible mechanism involved. Our results indicated that SPEE contained flavonoid, phenolic, and carotenoid as the major active constituents. The HPLC chromatogram revealed C-phycocyanin and fucoidan presented in SPEE. SPEE demonstrated the antioxidant capability and significantly reduced wound space at 24 and 48 h in wound-healing assay. Treatment with SPEE (50 and 100 μg/mL) increased FAK and Src phosphorylation in western blotting. Moreover, SPEE also upregulated Akt and p38 MAPK phosphorylation but not ERK1/2. SPEE increased Rac1 protein expression. Interestingly, hyaluronan synthase (HAS1 and HAS2) as well as collagen type I and elastin were also significantly upregulated when compared with the control upon exposure to SPEE. In conclusion, our data suggested that SPEE promotes cutaneous wound healing by regulating FAK/Src-mediated Akt, p38 MAPK, and Rac1 signaling. These findings suggest the potential use of SPEE for skin wound treatment.
最近，人们发现海藻提取物对皮肤有益。因此，本研究旨在探讨褐藻乙醇提取物（SPEE）对人皮肤角质形成细胞HaCaT细胞的植物化学分析、抗菌、抗氧化和伤口愈合特性以及其中可能涉及的机制。我们的结果表明，SPEE含有黄酮类、酚类和类胡萝卜素作为主要活性成分。HPLC色谱图显示SPEE中存在C-藻蓝蛋白和岩藻依聚糖。在伤口愈合试验中，SPEE表现出抗氧化能力，并在24小时和48小时时显著缩小伤口面积。在蛋白质免疫印迹法中，用SPEE（50和100μg/mL）处理可增加粘着斑激酶（FAK）和Src的磷酸化。此外，SPEE还上调了Akt和p38丝裂原活化蛋白激酶（MAPK）的磷酸化，但未上调细胞外信号调节激酶1/2（ERK1/2）。SPEE增加了Rac1蛋白的表达。有趣的是，与对照组相比，在暴露于SPEE后，透明质酸合成酶（HAS1和HAS2）以及I型胶原蛋白和弹性蛋白也显著上调。总之，我们的数据表明，SPEE通过调节FAK/Src介导的Akt、p38 MAPK和Rac1信号通路促进皮肤伤口愈合。这些发现表明SPEE在皮肤伤口治疗中的潜在应用价值。
