用快速放射测量法检测丝状真菌对两性霉素B的敏感性
Susceptibility testing of filamentous fungi to amphotericin B by a rapid radiometric method.
作者信息
Merz W G, Fay D, Thumar B, Dixon D
出版信息
J Clin Microbiol. 1984 Jan;19(1):54-6. doi: 10.1128/jcm.19.1.54-56.1984.
Abstract
A rapid, radiometric method was developed to determine the susceptibility of filamentous fungi to amphotericin B. The rapid, radiometric method depended on measurement of the inhibition of 14CO2 production in the presence of amphotericin B. Thirty isolates of filamentous fungi were tested by the rapid, radiometric method and a reference agar dilution method. There was 93% agreement between the two methods when an 80% or greater decrease in CO2 production was used to calculate the minimal inhibitory concentration with the rapid, radiometric method. Minimal inhibitory concentrations, based on 80% decrease of CO2 production, were achieved within 24 h of incubation with all of the fungi tested.
摘要
开发了一种快速放射性方法来测定丝状真菌对两性霉素B的敏感性。这种快速放射性方法依赖于在两性霉素B存在的情况下测量14CO2产生的抑制情况。用快速放射性方法和参考琼脂稀释法对30株丝状真菌进行了测试。当用快速放射性方法以二氧化碳产生减少80%或更多来计算最低抑菌浓度时，两种方法的一致性为93%。基于二氧化碳产生减少80%的最低抑菌浓度，在对所有测试真菌培养24小时内即可获得。
相似文献
1
Susceptibility testing of filamentous fungi to amphotericin B by a rapid radiometric method.用快速放射测量法检测丝状真菌对两性霉素B的敏感性
J Clin Microbiol. 1984 Jan;19(1):54-6. doi: 10.1128/jcm.19.1.54-56.1984.
2
Amphotericin B susceptibility testing of yeasts with a bactec radiometric system.采用Bactec放射测量系统对酵母进行两性霉素B药敏试验。
Antimicrob Agents Chemother. 1977 Feb;11(2):277-80. doi: 10.1128/AAC.11.2.277.
3
Effect of increasing inoculum sizes of pathogenic filamentous fungi on MICs of antifungal agents by broth microdilution method.通过肉汤微量稀释法增加致病丝状真菌接种量对抗真菌剂最低抑菌浓度的影响。
J Clin Microbiol. 1995 May;33(5):1302-7. doi: 10.1128/jcm.33.5.1302-1307.1995.
4
Antifungal activity of amphotericin B and itraconazole against filamentous fungi: comparison of the Sensititre Yeast One and NCCLS M38--a reference methods.两性霉素B和伊曲康唑对丝状真菌的抗真菌活性：Sensititre Yeast One与NCCLS M38参考方法的比较
J Chemother. 2004 Oct;16(5):468-73. doi: 10.1179/joc.2004.16.5.468.
5
Antifungal activities of posaconazole, ravuconazole, and voriconazole compared to those of itraconazole and amphotericin B against 239 clinical isolates of Aspergillus spp. and other filamentous fungi: report from SENTRY Antimicrobial Surveillance Program, 2000.泊沙康唑、雷夫康唑和伏立康唑与伊曲康唑及两性霉素B相比，对239株曲霉属及其他丝状真菌临床分离株的抗真菌活性：2000年SENTRY抗菌监测项目报告
Antimicrob Agents Chemother. 2002 Apr;46(4):1032-7. doi: 10.1128/AAC.46.4.1032-1037.2002.
6
Comparison of the Etest and the sensititre colorimetric methods with the NCCLS proposed standard for antifungal susceptibility testing of Aspergillus species.Etest法和比色法与美国国家临床实验室标准委员会（NCCLS）提出的曲霉属真菌药敏试验标准的比较。
J Clin Microbiol. 2002 Aug;40(8):2876-85. doi: 10.1128/JCM.40.8.2876-2885.2002.
7
In-vitro susceptibility testing by agar dilution method to determine the minimum inhibitory concentrations of amphotericin B, fluconazole and ketoconazole against ocular fungal isolates.采用琼脂稀释法进行体外药敏试验，以确定两性霉素B、氟康唑和酮康唑对眼部真菌分离株的最低抑菌浓度。
Indian J Med Microbiol. 2006 Oct;24(4):273-9. doi: 10.4103/0255-0857.29386.
8
Rapid radiometric method of testing susceptibility of mycobacteria and slow-growing fungi to antimicrobial agents.检测分枝杆菌和生长缓慢真菌对抗菌剂敏感性的快速放射测量法。
Antimicrob Agents Chemother. 1974 Jul;6(1):29-33. doi: 10.1128/AAC.6.1.29.
9
Comparison of the E-test with the NCCLS M38-P method for antifungal susceptibility testing of common and emerging pathogenic filamentous fungi.E-test法与NCCLS M38-P法用于常见和新出现的致病性丝状真菌抗真菌药敏试验的比较。
J Clin Microbiol. 2001 Apr;39(4):1360-7. doi: 10.1128/JCM.39.4.1360-1367.2001.
10
Overview of SPA-S-843 in vitro activity against filamentous fungi.SPA-S-843对丝状真菌的体外活性概述。
Chemotherapy. 2000 Jan-Feb;46(1):28-35. doi: 10.1159/000007253.
引用本文的文献
1
Potential application of a fungal co-culture crude extract for the conservation of post-harvest fruits.真菌共培养粗提物在采后水果保鲜中的潜在应用。
Braz J Microbiol. 2024 Jun;55(2):1679-1691. doi: 10.1007/s42770-024-01274-5. Epub 2024 Feb 23.
2
Rapid susceptibility testing of medically important zygomycetes by XTT assay.采用XTT法对医学上重要的接合菌进行快速药敏试验。
J Clin Microbiol. 2006 Feb;44(2):553-60. doi: 10.1128/JCM.44.2.553-560.2006.
3
Use of turbidimetric growth curves for early determination of antifungal drug resistance of filamentous fungi.使用比浊法生长曲线早期测定丝状真菌的抗真菌药物耐药性。
J Clin Microbiol. 2003 Oct;41(10):4718-25. doi: 10.1128/JCM.41.10.4718-4725.2003.
4
Antifungal susceptibility testing.抗真菌药敏试验
Clin Microbiol Rev. 1993 Oct;6(4):367-81. doi: 10.1128/CMR.6.4.367.
5
Potential use of BacT/Alert automated blood culture system for antifungal susceptibility testing.BacT/Alert自动血培养系统在抗真菌药敏试验中的潜在应用。
J Clin Microbiol. 1994 Mar;32(3):848-50. doi: 10.1128/jcm.32.3.848-850.1994.
6
Standardization of a hyphal inoculum of aspergilli for amphotericin B susceptibility testing.用于两性霉素B药敏试验的曲霉菌丝接种物标准化
J Clin Microbiol. 1985 Apr;21(4):509-12. doi: 10.1128/jcm.21.4.509-512.1985.
本文引用的文献
1
Rapid radiometric method of testing susceptibility of mycobacteria and slow-growing fungi to antimicrobial agents.检测分枝杆菌和生长缓慢真菌对抗菌剂敏感性的快速放射测量法。
Antimicrob Agents Chemother. 1974 Jul;6(1):29-33. doi: 10.1128/AAC.6.1.29.
2
Resistance to rifampicin and isoniazid in strains of Mycobacterium tuberculosis.结核分枝杆菌菌株对利福平和异烟肼的耐药性。
J Clin Pathol. 1981 Aug;34(8):927-9. doi: 10.1136/jcp.34.8.927.
3
Evaluation of rapid radiometric method for drug susceptibility testing of Mycobacterium tuberculosis.结核分枝杆菌药物敏感性检测快速放射测量法的评估
J Clin Microbiol. 1981 May;13(5):908-12. doi: 10.1128/jcm.13.5.908-912.1981.
4
Rapid drug-susceptibility testing of Mycobacterium tuberculosis.结核分枝杆菌的快速药敏试验
Am Rev Respir Dis. 1981 Apr;123(4 Pt 1):402-6. doi: 10.1164/arrd.1981.123.4.402.
5
Amphotericin B susceptibility testing of yeasts with a bactec radiometric system.采用Bactec放射测量系统对酵母进行两性霉素B药敏试验。
Antimicrob Agents Chemother. 1977 Feb;11(2):277-80. doi: 10.1128/AAC.11.2.277.
6
Antimicrobial susceptibility testing of six clinical isolates of Aspergillus.六种烟曲霉临床分离株的药敏试验
Antimicrob Agents Chemother. 1976 Jun;9(6):908-14. doi: 10.1128/AAC.9.6.908.
7
Radiometric method for determining the susceptibility of yeasts to 5-fluorocytosine.用于测定酵母对5-氟胞嘧啶敏感性的放射测量法。
Antimicrob Agents Chemother. 1979 Feb;15(2):313-4. doi: 10.1128/AAC.15.2.313.
8
Rapid radiometric susceptibility testing of Mycobacterium tuberculosis.结核分枝杆菌的快速放射药敏试验
Am Rev Respir Dis. 1978 Apr;117(4):631-7. doi: 10.1164/arrd.1978.117.4.631.
9
Determination of the minimal inhibitory concentration of amphotericin B and miconazole for 21 strains of Aspergillus.测定两性霉素B和咪康唑对21株曲霉菌的最低抑菌浓度。
Mycopathologia. 1978 Nov 10;64(3):147-51. doi: 10.1007/BF00576365.
Zotero 插件