Broich Lukas, Fu Yang, Sieben Christian
Nanoscale Infection Biology Group, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Institute of Genetics, Technische Universität Braunschweig, Braunschweig, Germany.
Methods Mol Biol. 2025;2890:89-101. doi: 10.1007/978-1-0716-4326-6_4.
Influenza A viruses are a major health care burden, and their biology has been intensely studied for decades. However, many details of virus infection are still elusive, requiring the development of refined and advanced technologies. Super-resolution microscopy allows the study of virus replication at the scale of an infecting virus, offering an exciting perspective on previously unseen mechanistic details of infection. Here we describe the materials and procedures required to perform single-molecule imaging of virus-receptor interaction in live cells. We further provide hints and tips on how to analyze and visualize the obtained datasets.
甲型流感病毒是一个重大的医疗负担，几十年来人们对其生物学特性进行了深入研究。然而，病毒感染的许多细节仍然难以捉摸，这需要开发精细和先进的技术。超分辨率显微镜能够在感染病毒的尺度上研究病毒复制，为以前未见的感染机制细节提供了一个令人兴奋的视角。在这里，我们描述了在活细胞中对病毒-受体相互作用进行单分子成像所需的材料和程序。我们还提供了有关如何分析和可视化所得数据集的提示和技巧。
