Li Yuwei, Liu Hao, Wang Xiaoyu, Wang Bo
College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao, Shandong, People's Republic of China.
College of Horticulture, Ludong University, Yantai, Shandong, People's Republic of China.
Mol Biol Rep. 2025 Feb 3;52(1):186. doi: 10.1007/s11033-025-10243-6.
The filamentation temperature-sensitive H (FtsH) gene family, which is known to play a critical role in plant growth and development by regulating photosynthesis, chloroplast development, and response to plant stress, has been extensively studied in various species. However, the FtsH gene family in wheat has not been previously documented.
In this study, 38 TaFtsH gene family members were identified, divided into eight groups and unevenly distributed across various chromosomes. Analysis of gene structure and conserved motifs revealed that TaFtsH genes within the same taxon share similar gene structures and conserved motifs. Further collinearity analysis provided insights into the evolutionary history of TaFtsH genes. Examination of cis-acting elements in the promoter region of TaFtsH genes revealed the presence of developmental and stress response elements in genes. The expression pattern of the wheat FtsH gene under various abiotic stresses was analyzed using real-time fluorescence quantitative PCR. Additionally, transient expression in tobacco verified the localization of the TaFtsH11-B protein in chloroplasts.
These findings collectively contribute to laying the groundwork for the functional characterization of TaFtsH genes.
丝状温度敏感H（FtsH）基因家族通过调节光合作用、叶绿体发育以及对植物胁迫的响应，在植物生长和发育中发挥关键作用，已在多种物种中得到广泛研究。然而，小麦中的FtsH基因家族此前尚未见报道。
在本研究中，鉴定出38个TaFtsH基因家族成员，分为八组，在不同染色体上分布不均。对基因结构和保守基序的分析表明，同一分类群内的TaFtsH基因具有相似的基因结构和保守基序。进一步的共线性分析为TaFtsH基因的进化历史提供了见解。对TaFtsH基因启动子区域顺式作用元件的检测揭示了基因中存在发育和胁迫响应元件。利用实时荧光定量PCR分析了小麦FtsH基因在各种非生物胁迫下的表达模式。此外，在烟草中的瞬时表达验证了TaFtsH11-B蛋白在叶绿体中的定位。
这些发现共同为TaFtsH基因的功能表征奠定了基础。
