血浆微小RNA-145-5p作为急性深静脉血栓形成的诊断生物标志物

Plasma microRNA-145-5p as a diagnostic biomarker for acute deep vein thrombosis.

作者信息

Antoun Christopher, Wikan Vårin Eiriksdatter, Øverli Øyvind, Ueland Thor, Yeganeh Gholamreza Jafari, Brækkan Sigrid Kufaas, Brodin Ellen, Hansen John-Bjarne

机构信息

Thrombosis Research Group, Department of Clinical Medicine, UiT-The Arctic University of Norway, Tromsø, Norway.

Division of Internal Medicine, Department of Haematology, Akershus University Hospital, Lørenskog, Norway.

出版信息

Res Pract Thromb Haemost. 2024 Dec 31;9(1):102671. doi: 10.1016/j.rpth.2024.102671. eCollection 2025 Jan.

DOI:10.1016/j.rpth.2024.102671

PMID:39902097

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11788850/

Abstract

BACKGROUND

Identification of new biomarkers for acute deep vein thrombosis (DVT) that could lower the need for diagnostic imaging to confirm or rule out the diagnosis would be advantageous. microRNA-145-5p (miR-145) has the potential to be a diagnostic marker for acute DVT, but its diagnostic performance has not been evaluated in consecutive patients referred to the hospital with suspected DVT.

OBJECTIVES

The aim of this study was to assess the diagnostic performance of miR-145 for the diagnosis of acute lower extremity DVT.

METHODS

Patients consecutively referred to the emergency room at Akershus University Hospital (Norway) due to suspicion of acute DVT between June 2021 and July 2023 were included. Within 24 hours of admission, blood was collected for assessment of plasma miR-145 (index test), and whole-leg compression ultrasound (reference standard) was used to confirm or rule out DVT. Cohen's d effect size and area under the receiver operating curve (AUC) were used to estimate the diagnostic performance of miR-145 and D-dimer.

RESULTS

Among 360 included patients, 101 (28%) were diagnosed with DVT. miR-145 showed poor diagnostic performance, as indicated by a Cohen's d of -0.002 (95% CI, -0.241 to 0.216) and an AUC of 0.59 (95% CI, 0.51-0.67). For D-dimer, Cohen's d was 1.66 (95% CI, 1.43-1.89), and the AUC was 0.92 (95% CI, 0.86-0.96).

CONCLUSION

Plasma levels of miR-145 showed poor diagnostic performance for lower extremity acute DVT among persons referred to the emergency room with clinical signs and symptoms of DVT.

摘要

背景

识别急性深静脉血栓形成（DVT）的新生物标志物，以减少用于确诊或排除诊断的诊断性成像需求，将是有益的。微小RNA-145-5p（miR-145）有可能成为急性DVT的诊断标志物，但其诊断性能尚未在因疑似DVT转诊至医院的连续患者中进行评估。

目的

本研究旨在评估miR-145对急性下肢DVT的诊断性能。

方法

纳入2021年6月至2023年7月期间因疑似急性DVT连续转诊至挪威阿克什胡斯大学医院急诊室的患者。入院后24小时内，采集血液以评估血浆miR-145（指标检测），并使用全腿压迫超声（参考标准）来确诊或排除DVT。采用科恩d效应量和受试者工作特征曲线下面积（AUC）来评估miR-145和D-二聚体的诊断性能。

结果

在360例纳入患者中，101例（28%）被诊断为DVT。miR-145的诊断性能较差，科恩d为-0.002（95%CI，-0.241至0.216），AUC为0.59（95%CI，0.51-0.67）。对于D-二聚体，科恩d为1.66（95%CI，1.43-1.89），AUC为0.92（95%CI，0.86-0.96）。