Oliveira Laisa Kindely Ramos de, Nascimento Neto Conrado Dias do, Costa E Silva Amy Brian, Rocha Stela Maris Wanderley, Bianchi Patrícia Roccon, Galdino André Gustavo de Sousa, Silva Daniela Nascimento
Postgraduate Program in Dental Sciences, UFES, Vitória, ES, Brazil.
, Marechal Campos, 1.355, Bonfim, Vitória, Espírito Santo, 29047160, Brazil.
Clin Oral Investig. 2025 Feb 4;29(2):108. doi: 10.1007/s00784-025-06195-7.
To carry out physicomechanical characterization of the HA/DCPA/TiO and to evaluate the tissue repair in rat calvaria.
Two bone defects were made in the calvaria of 36 Wistar rats, divided into groups: HA/DCPA, HA/DCPA/TiO and sham (blood clot). The animals were euthanized at 30, 60 and 90 days and calvaria slides were processed with hematoxylin/eosin. The newly formed bone, connective tissue, biomaterial remnant, and total tissue repair percentages were calculated in relation to the total defect area. The HA/DCPA/TiO was characterized structurally by scanning electron microscopy (SEM), and chemically by energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). It was submitted to apparent density (AD), apparent porosity (AP), water absorption (WA) and compressive strength (CS) physical tests. The ANOVA test was applied, followed by Turkey's test and Student's t-test (p ≤ 0,05).
The SEM showed biomaterials inside the bone defects and newly formed bone. EDS identified oxygen, calcium, phosphorus, and titanium in the sample. The HA/DCPA/TiO and HA/DCPA groups presented a total tissue repair area that was larger than the sham group (p < 0.001).
The physical-mechanical assays showed that HA/DCPA/TiO has AD and CS properties within the limits of trabecular bone and with values higher than HA/DCPA.HA/DCPA/TiO presented higher densification and compressive strength rates than HA/DCPA.
Potential as a scaffold for bone.
对HA/DCPA/TiO进行物理力学特性表征，并评估其在大鼠颅骨中的组织修复情况。
在36只Wistar大鼠的颅骨上制造两个骨缺损，分为HA/DCPA组、HA/DCPA/TiO组和假手术组（血凝块）。在30天、60天和90天时对动物实施安乐死，并将颅骨切片用苏木精/伊红染色处理。计算新形成的骨组织、结缔组织、生物材料残余物以及相对于总缺损面积的总组织修复百分比。通过扫描电子显微镜（SEM）对HA/DCPA/TiO进行结构表征，通过能量色散X射线光谱（EDS）和X射线衍射（XRD）进行化学表征。对其进行表观密度（AD）、表观孔隙率（AP）、吸水率（WA）和抗压强度（CS）物理测试。应用方差分析测试，随后进行Turkey检验和学生t检验（p≤0.05）。
SEM显示骨缺损内部有生物材料和新形成的骨组织。EDS在样品中鉴定出氧、钙、磷和钛。HA/DCPA/TiO组和HA/DCPA组的总组织修复面积大于假手术组（p<0.001）。
物理力学分析表明，HA/DCPA/TiO的AD和CS特性在松质骨范围内，且值高于HA/DCPA。HA/DCPA/TiO的致密化率和抗压强度率高于HA/DCPA。
具有作为骨支架的潜力。
