Kim Byurira, Lee Yong-Hyuk, Kim Ik-Hwan, Lee Ko Eun, Kang Chung-Min, Lee Hyo-Seol, Choi Hyung-Jun, Cheon Kyounga, Song Je Seon, Shin Yooseok
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Republic of Korea.
Oral Science Research Center, College of Dentistry, Yonsei University, Seoul, Republic of Korea.
J Dent Sci. 2023 Jul;18(3):1189-1198. doi: 10.1016/j.jds.2022.10.004. Epub 2022 Oct 20.
BACKGROUND/PURPOSE: As calcium silicate cements (CSCs) have been successfully used in various types of vital pulp therapy, many new CSC products have been developed. The aim of this study was to evaluate the biocompatibilities and mineralization potential of new CSC. The experimental materials were NeoMTA Plus and EndoSequence Root Repair Material-Fast Set Putty (ERRM-FS) which were compared to ProRoot MTA.
, the effects of the new CSC on stem cells were evaluated. Each CSC was prepared for cell viability testing, alkaline phosphatase (ALP) assay, and calcium ion release assay. the exposed pulp model was used for the partial pulpotomy procedure. Thirty-six teeth were treated with three materials: ProRoot MTA, NeoMTA Plus, or ERRM-FS. After four weeks, the teeth were extracted and processed for histologic analysis. Dentin bridge formation, pulp inflammation, and odontoblastic cell layer were evaluated and the area of newly formed calcific barrier of each group was measured.
Three CSCs demonstrated similar cell viability on stem cells and the levels of ALP and calcium release were not significantly different between tested materials. ProRoot MTA and ERRM-FS showed better tissue healing process than NeoMTA Plus after partial pulpotomy, in terms of quality of calcific barrier and pulp inflammation. The outcomes from measuring newly formed calcific area demonstrated no significant differences between the materials.
NeoMTA Plus and ERRM-FS displayed similar biocompatibilities and mineralization potential compared to ProRoot MTA. Therefore, these new CSCs can be used as desirable alternatives to ProRoot MTA.
背景/目的：由于硅酸钙水门汀（CSCs）已成功应用于各类活髓治疗，许多新型CSC产品不断涌现。本研究旨在评估新型CSC的生物相容性和矿化潜力。实验材料为NeoMTA Plus和EndoSequence Root Repair Material-Fast Set Putty（ERRM-FS），并与ProRoot MTA进行比较。
评估新型CSC对干细胞的影响。每种CSC均用于细胞活力测试、碱性磷酸酶（ALP）测定和钙离子释放测定。采用暴露牙髓模型进行部分牙髓切断术。36颗牙齿分别用三种材料处理：ProRoot MTA、NeoMTA Plus或ERRM-FS。四周后，拔除牙齿并进行组织学分析。评估牙本质桥形成、牙髓炎症和成牙本质细胞层，并测量每组新形成钙化屏障的面积。
三种CSC对干细胞的细胞活力相似，测试材料之间的ALP水平和钙释放量无显著差异。在部分牙髓切断术后，就钙化屏障质量和牙髓炎症而言，ProRoot MTA和ERRM-FS的组织愈合过程比NeoMTA Plus更好。新形成钙化面积的测量结果显示，材料之间无显著差异。
与ProRoot MTA相比，NeoMTA Plus和ERRM-FS表现出相似的生物相容性和矿化潜力。因此，这些新型CSC可作为ProRoot MTA的理想替代品。
