Dadlani Lakshmi Paloma, Gebiola Marco, Rugman-Jones Paul, Mauck Kerry E, Hoddle Mark S
Department of Entomology, University of California, Riverside, Riverside, CA, USA.
Department of Agricultural Sciences, University of Naples Federico II, Italy.
J Econ Entomol. 2025 Apr 26;118(2):884-894. doi: 10.1093/jee/toaf021.
Pseudacysta perseae (Heidemann 1908) (Hemiptera: Tingidae), a foliar pest of avocados, was first discovered in urban San Diego County, California U.S. in 2004. In 2017, damaging populations of P. perseae were discovered in commercial Hass avocado groves in San Diego and Riverside Counties. The unexpected and rapid spread of P. perseae raised the question of whether this range expansion was an extension of the original incursion or the result of a second invasion event. Using sequences of the mitochondrial COI gene, we found strong evidence that P. perseae populations in southern San Diego County are comprised almost entirely of the single haplotype (A) that was detected in 2004. In contrast, newly established (2017 onwards) populations of P. perseae infesting commercial orchards consisted exclusively of a second mitochondrial haplotype (G). This haplotype is found in Florida and the Caribbean and is conclusive evidence that California has been invaded a second time. Molecular analyses also confirmed that invasive P. perseae populations in Hawai'i are haplotype G, indicating that California and Hawai'i populations may have originated from the same source area (possibly Florida), or that California may have acted as an invasion bridgehead for the invasion of Hawai'i. Reproductive compatibility of the primary (haplotype A) and secondary (haplotype G) invasive populations in California was confirmed via a series of reciprocal laboratory crosses and results suggest that fertility of hybrid eggs is increased. The potential consequences of this second invasion into California by P. perseae are discussed.
鳄梨伪囊肿叶蝉（Pseudacysta perseae）（半翅目：叶蝉科）是鳄梨的一种叶部害虫，于2004年首次在美国加利福尼亚州圣地亚哥县的市区被发现。2017年，在圣地亚哥县和里弗赛德县的商业哈斯鳄梨果园中发现了造成危害的鳄梨伪囊肿叶蝉种群。鳄梨伪囊肿叶蝉意外且迅速的扩散引发了一个问题，即这种范围扩张是原始入侵的延伸，还是第二次入侵事件的结果。通过线粒体细胞色素氧化酶亚基I（COI）基因序列，我们发现有力证据表明，圣地亚哥县南部的鳄梨伪囊肿叶蝉种群几乎完全由2004年检测到的单一单倍型（A）组成。相比之下，2017年起在商业果园中出现的新的鳄梨伪囊肿叶蝉种群仅由第二种线粒体单倍型（G）组成。这种单倍型在佛罗里达州和加勒比地区被发现，是加利福尼亚州已被第二次入侵的确凿证据。分子分析还证实，夏威夷的入侵性鳄梨伪囊肿叶蝉种群也是单倍型G，这表明加利福尼亚州和夏威夷州的种群可能源自同一来源地区（可能是佛罗里达州），或者加利福尼亚州可能充当了夏威夷州入侵的桥头堡。通过一系列相互的实验室杂交，证实了加利福尼亚州主要（单倍型A）和次要（单倍型G）入侵种群的生殖兼容性，结果表明杂交卵的育性有所提高。本文讨论了鳄梨伪囊肿叶蝉第二次入侵加利福尼亚州的潜在后果。
