Galibert F, Mandart E, Fitoussi F, Tiollais P, Charnay P
Nature. 1979 Oct 25;281(5733):646-50. doi: 10.1038/281646a0.
The complete nucleotide sequence of hepatitis B virus genome (subtype ayw) cloned in Escherichia coli has been determined using the Maxam and Gilbert method and the dideoxynucleotide method. This sequence is 3,182 nucleotides long. Location of the nonsense codons shows that the coding capacity of the L chain is larger than the coding capacity of the S chain. Eight open regions, able to code for polypeptide chains larger than 100 amino acids, have been located. Region 6, which is the largest, covers more than 80% of the genome. The gene S which codes for polypeptide I of the Hbs Ag and was previously located between coordinates 95.1 and 73.6 is contained in region 7.
采用马克萨姆和吉尔伯特法以及双脱氧核苷酸法，已确定在大肠杆菌中克隆的乙型肝炎病毒基因组（ayw亚型）的完整核苷酸序列。该序列长3182个核苷酸。无义密码子的位置表明L链的编码能力大于S链的编码能力。已定位出八个开放区域，能够编码长度超过100个氨基酸的多肽链。最大的区域6覆盖了基因组的80%以上。编码乙肝表面抗原多肽I且先前位于坐标95.1和73.6之间的基因S包含在区域7中。
