Soares Kilmer Oliveira, Da Rocha Thamara Ferreira, Hale Vanessa L, Vasconcelos Priscylla Carvalho, do Nascimento Letícia José, da Silva Núbia Michelle Vieira, Rodrigues Adriana Evangelista, de Oliveira Celso José Bruno
Department of Animal Science, College of Agricultural Sciences (CCA), Federal University of Paraíba (UFPB), Areia, 58397-000, Paraiba, Brazil.
Department of Preventive Veterinary Medicine, Ohio State University, Coffey Rd., Columbus, OH, 43210, USA.
Sci Rep. 2025 Feb 12;15(1):5293. doi: 10.1038/s41598-025-85114-y.
The composition of the gut microbiota in animals can be influenced by a variety of intrinsic and extrinsic factors in the host, such as diet, physiological state, and genetics. This study aimed to compare the structural composition of the gut microbiota of Apis mellifera bees from two distinct Brazilian biomes, the Atlantic Forest and the Caatinga, using high throughput 16 S rRNA sequencing. We identified a core microbiota composed of seven genera present in all samples: Lactobacillus, Commensalibacter, Rhizobiaceae, Snodgrassella, Gilliamella, Orbaceae and Bifidobacterium. These taxa accounted for 63% of all bacterial genera in the dataset. Interestingly, we observed a significantly differential abundance of the genus Apibacter between bees from the two biomes, with a marked increase in bees from Atlantic Forest. However, the overall variance in the gut structural composition attributable to landscape type, while significant, was relatively low. Notably, none of the members of the core microbiota were differently abundant between the biomes. Understanding the magnitude of landscape-associated effects on the microbiota of bees in different biomes is crucial for the accurate assessment of the impact of anthropogenic factors. These findings provide important insights into the resilience and adaptability of the honey bee gut microbiome across contrasting environments, contributing to the development of conservation and sustainable management strategies for these essential pollinators.
动物肠道微生物群的组成会受到宿主中各种内在和外在因素的影响，如饮食、生理状态和基因。本研究旨在通过高通量16S rRNA测序比较来自巴西两个不同生物群落——大西洋森林和卡廷加的西方蜜蜂肠道微生物群的结构组成。我们鉴定出一个核心微生物群，由所有样本中均存在的七个属组成：乳酸杆菌属、共生杆菌属、根瘤菌科、斯氏菌属、吉氏菌属、 Orbaceae和双歧杆菌属。这些分类群占数据集中所有细菌属的63%。有趣的是，我们观察到来自两个生物群落的蜜蜂中Apibacter属的丰度存在显著差异，大西洋森林的蜜蜂中该属丰度显著增加。然而，归因于景观类型的肠道结构组成的总体差异虽然显著，但相对较低。值得注意的是，核心微生物群的成员在不同生物群落之间的丰度没有差异。了解不同生物群落中景观对蜜蜂微生物群的影响程度对于准确评估人为因素的影响至关重要。这些发现为蜜蜂肠道微生物群在不同环境中的恢复力和适应性提供了重要见解，有助于为这些重要传粉者制定保护和可持续管理策略。
