Asghar Muhammad Sohail, Ghazanfar Uzma, Rizwan Muhammad, Manan Muhammad Qasim, Baig Athar, Qaiser Muhammad Adnan, Haq Zeenat, Wang Lei, Duta Liviu
Ministry of Education Key Laboratory of Green Preparation and Application for Functional Materials, School of Materials Science and Engineering, Hubei University, Wuhan 430062, China.
Department of Physics, University of Wah, Wah Cantt 47040, Pakistan.
Int J Mol Sci. 2025 Jan 27;26(3):1095. doi: 10.3390/ijms26031095.
The treatment of bone cancer often necessitates the surgical removal of affected tissues, with artificial implants playing a critical role in replacing lost bone structure. Functionalized implants represent an innovative approach to improve bio-integration and the long-term effectiveness of surgery in treating cancer-damaged bones. In this study, nickel-substituted hydroxyapatite (Ni:HAp) nanoparticles were deposited as thin films using laser pulses in the range of 30,000-60,000. Comprehensive structural, infrared, optical, morphological, surface, and magnetic evaluations were conducted on the synthesized Ni:HAp thin films. The magnetic hysteresis (M-H) loop demonstrated an increase in the saturation magnetization of the films with a higher number of laser pulses. A minimum squareness ratio of 0.7 was observed at 45,000 laser pulses, and the M-H characteristics indicated a shift toward ferromagnetic behavior, achieving the desired thermal response through an alternating magnetic field application within 80 s. Thermogravimetric analysis revealed distinct thermal stability, with the material structure exhibiting 46% degradation at 800 °C. The incorporation of bioactive magnetic nanoparticles in the thin film holds significant promise for magnetic hyperthermia treatment. Using HDOCK simulations, the interactions between ligand molecules and proteins were also explored. Strong binding affinities with a docking score of -67.73 were thus observed. The presence of Ca ions enhances electrostatic interactions, providing valuable insights into the biochemical roles of the ligand in therapeutic applications. Intravenous administration of magnetic nanoparticles, which subsequently aggregate within the tumor tissue, combined with an applied alternating magnetic field, enable targeted heating of the tumor to 45 °C. This focused heating approach selectively targets cancer cells while preserving the surrounding healthy tissue, thereby potentially enhancing the effectiveness of hyperthermia therapy in cancer treatment.
骨癌的治疗通常需要手术切除受影响的组织，人工植入物在替代失去的骨骼结构方面起着关键作用。功能化植入物是一种创新方法，可改善生物整合以及手术治疗癌症损伤骨骼的长期效果。在本研究中，使用30000 - 60000范围内的激光脉冲将镍取代的羟基磷灰石（Ni:HAp）纳米颗粒沉积为薄膜。对合成的Ni:HAp薄膜进行了全面的结构、红外、光学、形态、表面和磁性评估。磁滞（M - H）回线表明，随着激光脉冲数量增加，薄膜的饱和磁化强度增加。在45000个激光脉冲时观察到最小矩形比为0.7，并且M - H特性表明向铁磁行为转变，通过在80秒内施加交变磁场实现了所需的热响应。热重分析显示出明显的热稳定性，材料结构在800℃时降解46%。在薄膜中掺入生物活性磁性纳米颗粒对磁热疗具有重要前景。使用HDOCK模拟，还探索了配体分子与蛋白质之间的相互作用。因此观察到具有-67.73对接分数的强结合亲和力。钙离子的存在增强了静电相互作用，为配体在治疗应用中的生化作用提供了有价值的见解。静脉注射磁性纳米颗粒，其随后在肿瘤组织内聚集，与施加的交变磁场相结合，能够将肿瘤靶向加热至45℃。这种聚焦加热方法在保留周围健康组织的同时选择性地靶向癌细胞，从而有可能提高热疗在癌症治疗中的有效性。
