Deng Siqi, Ma Wenjing, Cui Chunxu, Wang Shiqian, Jiang Mei
Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Ji'nan 250014, China.
Shandong Engineering Research Center for Innovation and Application of General Technology for Separation of Natural Products, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Ji'nan 250014, China.
Plants (Basel). 2025 Jan 24;14(3):348. doi: 10.3390/plants14030348.
is a herbaceous plant that possesses significant medicinal value. Land salinization affects the growth of , resulting in a decline in its quality and yield. Knotted1-like homeobox (KNOX) genes are transcription factors that play important roles in plant growth and abiotic stress. The characteristics and functions of KNOX genes in remain unclear. Here, we identified ten KNOX genes in , all of which possess the characteristic four domains: KNOX1, KNOX2, ELK, and HD. These s were divided into two groups together with homologous genes. Cis-acting element analysis indicated all s contained elements associated with phytohormone, light, and stress response. The s show tissue-specific expression among roots, stems, leaves, and flowers. We assessed the response of the s to salt stress using quantitative RT-PCR analysis. Notably, 4 expression significantly decreased within 24 h of salt exposure, while 1, 2, SmKNOX3, 8, and 9 showed significant increases. The expression of 1, 2, and 3 was significantly positively correlated with that of their target genes, 1 and S11 . These findings suggest that s and their target genes respond to salt stress, providing a foundation for studies of s and the potential genetic improvement of .
是一种具有重要药用价值的草本植物。土壤盐渍化影响其生长,导致其品质和产量下降。类结瘤素1同源异型框(KNOX)基因是在植物生长和非生物胁迫中起重要作用的转录因子。该植物中KNOX基因的特征和功能尚不清楚。在此,我们在该植物中鉴定出10个KNOX基因,它们均具有KNOX1、KNOX2、ELK和HD这四个特征结构域。这些基因与同源基因一起被分为两组。顺式作用元件分析表明,所有基因都含有与植物激素、光和胁迫反应相关的元件。这些基因在根、茎、叶和花中表现出组织特异性表达。我们使用定量RT-PCR分析评估了这些基因对盐胁迫的反应。值得注意的是,4号基因在盐处理24小时内表达显著下降,而1、2、SmKNOX3、8和9号基因则显著上调。1、2和3号基因的表达与其靶基因1和S11的表达显著正相关。这些发现表明,该植物中的KNOX基因及其靶基因对盐胁迫有反应,为该植物中KNOX基因的研究及其潜在的遗传改良提供了基础。