Miller T L, Wolin M J
Arch Microbiol. 1985 Mar;141(2):116-22. doi: 10.1007/BF00423270.
Methanosphaera stadtmaniae is a non-motile, Gram-positive spherical-shaped organism that obtains energy for growth by using hydrogen to reduce methanol to methane. It does not produce methane from hydrogen and carbon dioxide, formate, acetate or methylamines and cannot grow with hydrogen and carbon monoxide, nitrate, fumarate, sulfate or choline. Its pH optimum is 6.5 to 6.9 and its temperature optimum is 36 degrees to 40 degrees C. It is not inhibited by bile salts, inhibitors of the synthesis of folic acid coenzymes, cephalothin or clindamycin but is inhibited by metronidazole, bacitracin, monensin, lasalocid, or bromoethanesulfonate. It requires acetate, carbon dioxide, isoleucine, ammonium, and thiamin for growth and biotin is stimulatory. It does not contain cytochromes and the mol % G + C of its DNA is 25.8. The composition of its cell wall and 16 S rRNA and its immunological fingerprint are consistent with characterization of the organism as a member of a new genus of the family Methanobacteriaceae. The habitat of the type strain is the human large intestine.
史氏甲烷球形菌是一种无运动性、革兰氏阳性的球形微生物，它通过利用氢气将甲醇还原为甲烷来获取生长所需的能量。它不能利用氢气和二氧化碳、甲酸盐、乙酸盐或甲胺产生甲烷，也不能在氢气和一氧化碳、硝酸盐、富马酸盐、硫酸盐或胆碱存在的情况下生长。其最适pH值为6.5至6.9，最适温度为36摄氏度至40摄氏度。它不受胆盐、叶酸辅酶合成抑制剂、头孢噻吩或克林霉素的抑制，但受甲硝唑、杆菌肽、莫能菌素、拉沙洛西或溴乙烷磺酸盐的抑制。它的生长需要乙酸盐、二氧化碳、异亮氨酸、铵和硫胺素，生物素具有促进作用。它不含细胞色素，其DNA的G + C摩尔百分比为25.8。其细胞壁的组成、16S rRNA及其免疫指纹图谱与该微生物作为甲烷杆菌科一个新属成员的特征一致。模式菌株的栖息地是人类大肠。
