Miller T L, Wolin M J, Conway de Macario E, Macario A J
Appl Environ Microbiol. 1982 Jan;43(1):227-32. doi: 10.1128/aem.43.1.227-232.1982.
Fecal specimens from nine adults were examined for the presence of methanogenic bacteria. Enrichment cultures of five specimens produced methane in 5 days. Of these five specimens, three were tested and produced methane during a short-term incubation. Four specimens did not produce methane in either short-term incubation or in enrichment culture. Each methanogenic culture contained methanogens similar in morphology to organisms of the genus Methanobrevibacter and showed factor-420 fluorescence by fluorescence microscopy. Pure cultures were obtained from four of the five methanogenic enrichment cultures. Each isolate grew and formed methane from either H2-CO2 or formate, but growth obtained with formate was poor. None of the isolates used acetate, methanol, or trimethylamine. All isolates grew in the presence of bile salts. In immunological studies, each isolate was closely related to the type strain of Methanobrevibacter smithii, a finding consistent with the physiological and morphological similarities between the isolates and the type strain.
对9名成年人的粪便样本进行了产甲烷菌检测。5份样本的富集培养物在5天内产生了甲烷。在这5份样本中，有3份经过短期培养测试后产生了甲烷。另外4份样本在短期培养或富集培养中均未产生甲烷。每种产甲烷培养物中所含的产甲烷菌在形态上与短柄产甲烷杆菌属的微生物相似，通过荧光显微镜观察显示出420因子荧光。从5份产甲烷富集培养物中的4份获得了纯培养物。每株分离菌都能利用H₂-CO₂或甲酸盐生长并产生甲烷，但利用甲酸盐时生长较差。所有分离菌均不利用乙酸盐、甲醇或三甲胺。所有分离菌在胆盐存在的情况下都能生长。在免疫学研究中，每株分离菌都与史氏短柄产甲烷杆菌的模式菌株密切相关，这一发现与分离菌和模式菌株之间的生理及形态相似性相符。
