Samsonoff W A, Hashimoto T, Conti S F
J Bacteriol. 1970 Mar;101(3):1038-45. doi: 10.1128/jb.101.3.1038-1045.1970.
The sequence of events occurring during the germination and outgrowth of appendage-bearing spores of Clostridium bifermentans was studied by phase-contrast and electron microscopy. The mature spore was characterized ultrastructurally as having the normal spore components as well as long tubular appendages which orginated from the surface of the spore coat. Spores were incompletely enclosed by a distinctly laminated exosporium which possessed hairlike projections on its outermost layer. During germination, structural changes were observed in the core, core wall, cortex, and spore coat layers. Cortical material was extruded from the spore during outgrowth, which usually occurred from the pole opposite the appendages. The subunits comprising the structure of the appendages and the morphology of the mature appendages were observed. No discernible changes could be observed in the spore appendages during germination and outgrowth.
通过相差显微镜和电子显微镜研究了双酶梭菌带附属物孢子萌发和生长过程中发生的一系列事件。成熟孢子的超微结构特征是具有正常的孢子成分以及从孢子外壁表面长出的长管状附属物。孢子不完全被明显分层的芽孢外壁包围，芽孢外壁的最外层有毛发状突起。在萌发过程中，观察到核心、核心壁、皮层和孢子外壁层发生了结构变化。皮层物质在生长过程中从孢子中挤出，通常从与附属物相对的极部发生。观察了组成附属物结构的亚基和成熟附属物的形态。在萌发和生长过程中，孢子附属物未观察到明显变化。
