Geraghty D E, Messing J, Rubenstein I
EMBO J. 1982;1(11):1329-35. doi: 10.1002/j.1460-2075.1982.tb01318.x.
The nucleotide sequence of two zein cDNAs in hybrid plasmids A20 and B49 have been determined. The insert in A20 is 921 bp long including a 5' non-coding region of 60 nucleotides, preceded by what is believed to be an artifactual sequence of 41 nucleotides, and a 3' non-coding region of 87 nucleotides. The B49 insert is 467 bp long and includes approximately one-half the protein coding sequence as well as a 3' non-coding region of 97 nucleotides. These sequences have been compared with the previously published sequence of another zein clone, A30 . A20 and A30 , both encoding 19 000 mol. wt. zeins , have approximately 85% homology at the nucleotide level. The B49 sequence, corresponding to a 22 000 mol. wt. zein, has approximately 65% homology to either A20 or A30 . All three zeins share common features including nearly identical amino acid compositions. In addition, the tandem repeats of 20 amino acids first seen in A30 are also present in A20 and B49 .
已确定杂交质粒A20和B49中两个玉米醇溶蛋白cDNA的核苷酸序列。A20中的插入片段长921 bp，包括一个60个核苷酸的5'非编码区，其前面是一个据信为41个核苷酸的人为序列，以及一个87个核苷酸的3'非编码区。B49插入片段长467 bp，包括大约一半的蛋白质编码序列以及一个97个核苷酸的3'非编码区。这些序列已与先前发表的另一个玉米醇溶蛋白克隆A30的序列进行了比较。A20和A30都编码19000 mol.wt.的玉米醇溶蛋白，在核苷酸水平上具有大约85%的同源性。B49序列对应于一种22000 mol.wt.的玉米醇溶蛋白，与A20或A30大约有65%的同源性。所有三种玉米醇溶蛋白都具有共同特征，包括几乎相同的氨基酸组成。此外，首次在A30中发现的20个氨基酸的串联重复序列在A20和B49中也存在。
