Guo Shuangyuan, Zhang Feng, Du Xiaoya, Zhang Xinmei, Huang Xueling, Li Zelong, Zhang Yanqin, Gan Pengfei, Li Huankun, Li Min, Wang Xinyue, Tang Chunlei, Wang Xiaojie, Kang Zhensheng, Zhang Xinmei
College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling, Shaanxi 712100, China.
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China.
Dev Cell. 2025 Jun 23;60(12):1702-1718.e6. doi: 10.1016/j.devcel.2025.01.017. Epub 2025 Feb 14.
Nucleotide-binding site, leucine-rich repeat (NLR) proteins activate a robust immune response on recognition of pathogen invasion. However, the function and regulatory mechanisms of NLRs during Puccinia striiformis f. sp. tritici (Pst) infection in wheat remain elusive. Here, we identify an ankyrin (ANK) repeat and tetratricopeptide repeat (TPR)-containing protein, TaANK-TPR1, which plays a positive role in the regulation of wheat resistance against Pst and the immune response of NLR. TaANK-TPR1 targets the NLR protein TaRPP13L1 (Recognition of Peronospora Parasitica 13-like 1) to facilitate its homodimerization and cell death to enhance the resistance of wheat against Pst. Meanwhile, TaANK-TPR1 binds to the TGACGT motif (methyl jasmonate-responsive element) of the TaRPP13L1 promoter and activates TaRPP13L1 transcription. Both TaANK-TPR1 and TaRPP13L1 respond to jasmonic acid (JA) signaling via the TGACGT element. Importantly, overexpressing TaRPP13L1 confers robust rust resistance without impacting important agronomic traits in the field. These findings identify a regulatory mechanism of NLR protein and provide targets for improving crop disease resistance.
核苷酸结合位点富含亮氨酸重复序列（NLR）蛋白在识别病原体入侵时会激活强大的免疫反应。然而，NLRs在小麦条锈菌（Pst）感染过程中的功能和调控机制仍不清楚。在这里，我们鉴定了一种含有锚蛋白（ANK）重复序列和四肽重复序列（TPR）的蛋白TaANK-TPR1，它在调节小麦对Pst的抗性和NLR的免疫反应中发挥积极作用。TaANK-TPR1靶向NLR蛋白TaRPP13L1（寄生霜霉13样1识别蛋白），促进其同源二聚化和细胞死亡，以增强小麦对Pst的抗性。同时，TaANK-TPR1与TaRPP13L1启动子的TGACGT基序（茉莉酸甲酯响应元件）结合并激活TaRPP13L1转录。TaANK-TPR1和TaRPP13L1都通过TGACGT元件响应茉莉酸（JA）信号。重要的是，过表达TaRPP13L1可赋予强大的抗锈性，而不会影响田间重要农艺性状。这些发现确定了NLR蛋白的调控机制，并为提高作物抗病性提供了靶点。
