Chondamma Tanya, Cariappa Ponappa Muckatira, Ponnappa K C, Shashidara R
Department of Conservative Dentistry and Endodontics, Coorg Institute of Dental Sciences, Virajpet, Karnataka, India.
Department of Oral Pathology, Coorg Institute of Dental Sciences, Virajpet, Karnataka, India.
J Conserv Dent Endod. 2024 Dec;27(12):1280-1284. doi: 10.4103/JCDE.JCDE_644_24. Epub 2024 Dec 10.
Irrigation is a critical component in accessing the labyrinth of the pulp space. Although traditional irrigants such as sodium hypochlorite (NaOCl) are widely employed, they exhibit several limitations, including cytotoxicity and complete microbial eradication. In contrast, phytochemicals derived from plants, including coffee, present promising alternatives due to their established antimicrobial properties.
The aim of this study was to evaluate the efficacy of intracanal irrigants derived from locally cultivated and (Robusta) varieties, both recognized for their antimicrobial attributes.
A total of 70 extracted human single-rooted teeth were prepared. Coffee extracts were prepared through a decoction method, and the minimum inhibitory concentrations (MICs) of these extracts were determined using microbroth dilution assays. Microbial growth was quantitatively assessed at predetermined intervals.
Green Robusta extracts exhibited antimicrobial effectiveness at lower concentrations of 25% and 12.5%. All coffee extract groups demonstrated substantial reductions in microbial counts compared to the negative control, with Robusta extracts proving particularly efficacious. Notably, the Robusta green 12.5% extract exhibited sustained antimicrobial efficacy, suggesting its potential as an alternative to NaOCl.
The findings of this study suggest a novel approach to improving endodontic therapy outcomes through the utilization of coffee extracts, potentially enhancing dental health.
冲洗是进入牙髓腔复杂结构的关键环节。尽管次氯酸钠（NaOCl）等传统冲洗剂被广泛使用，但它们存在一些局限性，包括细胞毒性和无法完全消除微生物。相比之下，源自植物的植物化学物质，包括咖啡，因其已证实的抗菌特性而展现出有前景的替代方案。
本研究的目的是评估源自当地种植的罗布斯塔（Robusta）品种咖啡豆的根管冲洗剂的疗效，这两个品种均以其抗菌特性而闻名。
总共准备了70颗拔除的人类单根牙。通过煎煮法制备咖啡提取物，并使用微量肉汤稀释法测定这些提取物的最低抑菌浓度（MIC）。在预定的时间间隔对微生物生长进行定量评估。
绿色罗布斯塔提取物在25%和12.5%的较低浓度下表现出抗菌效果。与阴性对照相比，所有咖啡提取物组的微生物数量均大幅减少，罗布斯塔提取物尤其有效。值得注意的是，罗布斯塔绿色12.5%提取物表现出持续的抗菌效果，表明其有潜力替代NaOCl。
本研究结果表明，通过利用咖啡提取物改善牙髓治疗效果是一种新方法，可能会增强牙齿健康。
