Morgun E I, Sukhinich K K, Rogovaya O S, Vorotelyak E A
PhD, Researcher, Laboratory of Cellular Biology; Koltzov Institute of Developmental Biology of Russian Academy of Sciences, 26 Vavilov St., Moscow, 119334, Russia.
PhD, Researcher, Laboratory of Regeneration Problems; Koltzov Institute of Developmental Biology of Russian Academy of Sciences, 26 Vavilov St., Moscow, 119334, Russia.
Sovrem Tekhnologii Med. 2023;15(5):5-13. doi: 10.17691/stm2023.15.5.01. Epub 2023 Oct 30.
was to evaluate the possibility of using our ischemic non-healing wound model for preclinical studies of biomedical cell products (BCP) during transplantation of a tissue-engineered construct. were to conduct the experiment on the transplantation of the tissue-engineered construct "living skin equivalent" (LSE) and select methods for determining the effectiveness of treating ischemic non-healing wounds during preclinical studies on the proposed model.
The study was performed on 56 BALB/c mice divided into the following groups: "control" (n=19), "scaffold" (n=19), and "LSE" (n=18).During the experiment, the histological, immunohistochemical methods and raster scanning optoacoustic mesoscopy (RSOM) technique were employed to compare the dynamics of regeneration of ischemic non-healing wound using LSE transplantation, collagen-hyaluronic film as a cell scaffold, and a non-treated wound.
Histology and immunohistochemistry have been found to be suitable to assess the effectiveness of treating ischemic non-healing wounds during preclinical investigations. The effect of LSE transplantation on infiltration of the wound bed with inflammatory cells, the formation of tissue in the wound bed zone, tissue condition at the wound margins, and angiogenesis has been studied. In addition, a new smoothing coefficient, i.e. the ratio of the thickness of the tissue-remodeling zone to the thickness of the dermis of the wound margins, has been proposed in the study. This coefficient makes it possible to assess the degree of filling the wound bed with the developing tissue. Its high value in the LSE group means that BCP transplantation influences the granulation tissue growth, prevents mechanical stress in the wound preventing thereby cosmetic defects.Exploration of the regenerative processes has shown that the proposed model of the ischemic non-healing wound is suitable for preclinical studies of BCP.
目的是评估在组织工程构建体移植过程中，使用我们的缺血性不愈合伤口模型进行生物医学细胞产品（BCP）临床前研究的可能性。进行组织工程构建体“活性皮肤替代物”（LSE）移植实验，并选择在拟议模型的临床前研究中确定治疗缺血性不愈合伤口有效性的方法。
对56只BALB/c小鼠进行研究，分为以下几组：“对照组”（n = 19）、“支架组”（n = 19）和“LSE组”（n = 18）。实验过程中，采用组织学、免疫组织化学方法和光栅扫描光声显微镜（RSOM）技术，比较使用LSE移植、胶原 - 透明质酸膜作为细胞支架以及未处理伤口的缺血性不愈合伤口的再生动态。
已发现组织学和免疫组织化学适用于评估临床前研究中缺血性不愈合伤口的治疗效果。研究了LSE移植对伤口床炎性细胞浸润、伤口床区域组织形成、伤口边缘组织状况和血管生成的影响。此外，该研究提出了一个新的平滑系数，即组织重塑区厚度与伤口边缘真皮厚度之比。该系数能够评估伤口床被发育中的组织填充的程度。LSE组中的高值意味着BCP移植影响肉芽组织生长，防止伤口处的机械应力，从而预防外观缺陷。对再生过程的探索表明，所提出的缺血性不愈合伤口模型适用于BCP的临床前研究。
