Nematicidal potential of Sneb159 against and the complete genome sequence analysis.

INTRODUCTION

is one of the most important pathogens of soybean production worldwide. Biological control provides a strategy for sustainable and environmentally friendly nematode management.

METHODS

In this study, solid-phase microextraction gas chromatography-mass spectrometry (SPME-GC-MS) was used to reveal the volatile nematicidal compounds of Sneb159 and the mode of action was further elucidated via whole genome sequencing.

RESULTS

The present study demonstrated that Sneb159 fermentation broth showed strong nematicidal activities against . The filtrate rather than bacterial cells played a role in the nematicidal character, and reduced the invasion number as well as suppressed the development of juveniles in soybean. The analysis of chemotaxis showed that Sneb159 has a repellent effect on in pot experiments. The volatiles produced by Sneb159 are toxic to for both juveniles and eggs. The seven compounds were analyzed using SPME-GC-MS. In the bioassays, dimethyl disulfide and dimethyl trisulfide showed both direct contact and fumigated effect on juveniles and eggs. The complete genome sequence of the bacterium Sneb159 was completed using the PacBio sequencing platform. The genome comprised 3895529 bp and a 68.63% G + C content. Three secondary metabolites gene clusters were predicted by the antiSMASH system.

DISCUSSION

The findings reveal multifunction of Sneb159 towards , and has the potential to be developed as a safe nematicidal agent.