Zeybek Zuhal, Binay Ali Rıza
Istanbul University, Science Faculty, Biology Department, Fundamental and Industrial Microbiology Division 34134, Vezneciler - Istanbul, Turkey.
Istanbul University, Science Faculty, Biology Department, Fundamental and Industrial Microbiology Division 34134, Vezneciler - Istanbul, Turkey.
Exp Parasitol. 2014 Nov;145 Suppl:S121-6. doi: 10.1016/j.exppara.2014.06.009. Epub 2014 Jun 20.
When bacteria and free-living amoebae (FLAs) live both in natural waters and man-made aquatic systems, they constantly interact with each other. Some bacteria can survive and grow within FLAs. Therefore, it has recently been thought that FLAs play an important role in spreading pathogenic bacteria in aquatic systems. In this study we investigated the intracellular growing ability of 7 different Gram-negative bacteria (Pseudomonas fluorescens, Pseudomonas putida, Pasteurella pneumotropica, Aeromonas salmonicida, Legionella pneumophila serogroup 1, L. pneumophila serogroup 3, L. pneumophila serogroup 6) in four different FLA isolates (A1-A4). Among these, four bacterial isolates (P. fluorescens, P.putida, P.pneumotropica, A.salmonicida) and two free-living amoebae isolates (A3, A4) were isolated from the tap water in our city (Istanbul). It was found that 4 different Gram-negative bacteria could grow in A1, 2 different Gram-negative bacteria could grow in A2, 4 different Gram-negative bacteria could grow in A3, 1 Gram-negative bacterium could grow in A4. In conclusion, we think that this ability of growth could vary according to the characteristics of both bacteria and FLA isolates. Also, other factors such as environmental temperature, bacterial concentration, and extended incubation period may play a role in these interactions. This situation can be clarified with future studies.
当细菌与自由生活的变形虫（FLAs）共存于天然水体和人工水系统中时，它们会持续相互作用。一些细菌能够在FLAs内存活并生长。因此，近来人们认为FLAs在水系统中传播病原菌方面发挥着重要作用。在本研究中，我们调查了7种不同的革兰氏阴性菌（荧光假单胞菌、恶臭假单胞菌、嗜肺巴斯德杆菌、杀鲑气单胞菌、嗜肺军团菌血清群1、嗜肺军团菌血清群3、嗜肺军团菌血清群6）在4种不同的FLA分离株（A1 - A4）中的细胞内生长能力。其中，4种细菌分离株（荧光假单胞菌、恶臭假单胞菌、嗜肺巴斯德杆菌、杀鲑气单胞菌）和2种自由生活的变形虫分离株（A3、A4）是从我市（伊斯坦布尔）的自来水中分离得到的。结果发现，4种不同的革兰氏阴性菌能在A1中生长，2种不同的革兰氏阴性菌能在A2中生长，4种不同的革兰氏阴性菌能在A3中生长，1种革兰氏阴性菌能在A4中生长。总之，我们认为这种生长能力可能因细菌和FLA分离株的特性而异。此外，环境温度、细菌浓度和延长的孵育期等其他因素可能在这些相互作用中发挥作用。这种情况有待未来的研究加以阐明。
