Park Jung Min, Nam Gi Beag, Lee Eun-Soo, Kim Hyung-Min, Kim Hyuk, Myoung Kilsun, Lee Ji Ean, Baek Heung Soo, Ko Jaeyoung, Lee Chang Seok
Department of Senior Healthcare Major in Biopharmaceuticals, Eulji University, Sanseong-daero 553, Sujeong-gu, Seongnam-si, Gyeonggi-do, 13135, Republic of Korea.
AMOREPACIFIC Research and Innovation Center, 1920, Yonggu-daero, Giheung-gu, Yongin-si, Gyeonggi-do, 17074, Republic of Korea.
Arch Dermatol Res. 2025 Feb 24;317(1):483. doi: 10.1007/s00403-025-03885-w.
The skin acts as a crucial barrier and, upon injury, initiates complex wound-healing processes involving various cell types. Polydeoxyribonucleotides (PDRNs) are well-known for their efficacy in enhancing skin regeneration and wound healing. This study sought to investigate the effectiveness of PDRNs derived from Chlorella protothecoides, a sustainable and scalable microalgal source, in promoting skin regeneration and wound healing. Keratinocytes and fibroblasts were used for assessing the impact of PDRNs on cell proliferation, migration, collagen synthesis, and angiogenesis. Gene expression and associated signaling pathways were also examined using RT-qPCR and Western blot analyses. Our findings demonstrated that PDRNs significantly enhanced the proliferation and migration of skin cells, upregulated growth arrest specific 6 (GAS6) and hepatocyte growth factor (HGF) expression, and increased collagen synthesis by modulating collagen type I alpha 1 (COLIA1) expression. Additionally, PDRNs enhanced angiogenesis by promoting vascular endothelial growth factor (VEGF) expression and activation of ERK, AKT, β-catenin and STAT3 pathways via an adenosine A2A receptor (A2AR)-dependent mechanism. These findings suggest that microalgal-derived PDRNs have significant potential as sustainable and effective agents for clinical and cosmetic applications aimed at improving skin health and wound healing.
皮肤起着至关重要的屏障作用，受伤时会启动涉及多种细胞类型的复杂伤口愈合过程。聚脱氧核糖核苷酸（PDRNs）因其在促进皮肤再生和伤口愈合方面的功效而闻名。本研究旨在探讨源自原球藻（一种可持续且可扩展的微藻来源）的PDRNs在促进皮肤再生和伤口愈合方面的有效性。使用角质形成细胞和成纤维细胞来评估PDRNs对细胞增殖、迁移、胶原蛋白合成和血管生成的影响。还使用RT-qPCR和蛋白质印迹分析来检测基因表达和相关信号通路。我们的研究结果表明，PDRNs显著增强了皮肤细胞的增殖和迁移，上调了生长停滞特异性蛋白6（GAS6）和肝细胞生长因子（HGF）的表达，并通过调节I型胶原蛋白α1（COLIA1）的表达增加了胶原蛋白的合成。此外，PDRNs通过促进血管内皮生长因子（VEGF）的表达以及通过腺苷A2A受体（A2AR）依赖性机制激活ERK、AKT、β-连环蛋白和STAT3信号通路来增强血管生成。这些研究结果表明，源自微藻的PDRNs作为旨在改善皮肤健康和伤口愈合的临床和美容应用的可持续且有效的药物具有巨大潜力。
