Xiong Chengjie, Lin Yongsheng, Keyhani Nemat O, Shang Junya, Mao Yuchen, Yang Jiao, Zheng Minghai, Yang Lixia, Pu Huili, Lin Longbing, Mu Taichang, Zhu Mengjia, Wu Ziyi, Qiu Zhenxing, Xiong Wen, Guan Xiayu, Qiu Junzhi
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Department of Biological Sciences, University of Illinois, Chicago, IL 60607, USA.
J Fungi (Basel). 2025 Jan 24;11(2):94. doi: 10.3390/jof11020094.
Members of the genus (Hypocreales, Clavicipitaceae) are insect pathogens with specificity for scale insects and whiteflies. However, no mitochondrial genomes are available for these fungi. Here, we assembled seven mitogenomes from , , , , , sp. CGMCC 3.18909, and sp. CGMCC 3.18913, which varied in size from 40.8 to 95.7 Kb. Synteny and codon usage bias was relatively conserved, with the mitochondrial gene arrangement being completely homologous to the gene order of 21 other species within the Hypocreales. Nevertheless, significant intron polymorphism was observed between species. Evolutionary analyses revealed that all 15 core protein-coding genes had ka/ks < 1, indicating purifying selection pressure. Sequence variation within the mitochondrial ATP synthase F0 subunit 6 () gene showed the largest genetic distance, with the ATP synthase F0 subunit 9 () gene showing the smallest. Comparative analyses of mitogenomes revealed that introns were the primary factor contributing to the size variation in and, more broadly, within Hypocreales mitogenomes. Phylogenetic analyses indicated that the seven species examined form a well-supported clade, most closely related to . These data present the first mitogenomes from and further advance research into the taxonomy, origin, evolution, and genomics of .
（肉座菌目，麦角菌科）属的成员是对介壳虫和粉虱具有特异性的昆虫病原体。然而，这些真菌的线粒体基因组尚未可得。在此，我们组装了来自、、、、、CGMCC 3.18909菌种和CGMCC 3.18913菌种的七个有丝分裂基因组，其大小从40.8到95.7千碱基对不等。同线性和密码子使用偏好相对保守，线粒体基因排列与肉座菌目内其他21个物种的基因顺序完全同源。然而，在物种之间观察到了显著的内含子多态性。进化分析表明，所有15个核心蛋白质编码基因的ka/ks < 1，表明存在纯化选择压力。线粒体ATP合酶F0亚基6（）基因内的序列变异显示出最大的遗传距离，而ATP合酶F0亚基9（）基因显示出最小的遗传距离。有丝分裂基因组的比较分析表明，内含子是导致和更广泛地在肉座菌目有丝分裂基因组中大小变异的主要因素。系统发育分析表明，所检测的七个物种形成了一个得到充分支持的进化枝，与关系最为密切。这些数据展示了来自的首个有丝分裂基因组，并进一步推动了对的分类学、起源、进化和基因组学的研究。
