Povýsil C, Matĕjovský Z
Pathol Res Pract. 1985 Mar;179(4-5):546-59. doi: 10.1016/S0344-0338(85)80196-5.
The present study is based on electron microscopical examinations of 15 conventional chondrosarcomas, 1 clear cell chondrosarcoma, 3 mesenchymal chondrosarcomas, 2 so-called chordoid sarcomas (extraskeletal myxoid chondrosarcoma), 4 sacrococcygeal chordomas, 2 ecchordoses and 1 neoplasm of tibia with features of a true peripheral chordoma (parachordoma). The neoplastic cells from various types of chondrosarcoma shared a number of features with nonneoplastic chondrocytes as e.g. a well-developed rough endoplasmic reticulum and microvillous cytoplasmic processes. In clear-cell chondrosarcoma, glycogen accumulation in the tumour cells was a prominent feature. The cells of mesenchymal chondrosarcoma usually showed the characteristics of immature mesenchymal cells. In contrast, chordomas commonly contained physaliferous cells with two types of vacuoles in their cytoplasm. The first type can be most adequately characterized as intracytoplasmic pseudoinclusions of intercellular substance, whereas the other type, glycogen-containing, single membrane-bound vacuoles most probably correspond to autophagosomes (cytolysosomes). Only vacuoles of the first type were recorded in the so-called chordoid sarcoma. They were also seen in chondrosarcomas. In contrast, both types of vacuoles were identified in the above-mentioned tibial tumour which, in addition, showed even other cytological characteristics of chordoma. The findings presented here have demonstrated distinct structural relationships between chordoid sarcoma and chondrogenic tumours. On the other hand, our observation of the uncommon tibial neoplasm indicates the possibility that tumours identical with chordoma may occur at sites other than the axial skeleton.
本研究基于对15例传统型软骨肉瘤、1例透明细胞软骨肉瘤、3例间叶性软骨肉瘤、2例所谓的脊索样肉瘤（骨外黏液样软骨肉瘤）、4例骶尾部脊索瘤、2例脊索样肿物以及1例具有真性外周型脊索瘤（副脊索瘤）特征的胫骨肿瘤的电子显微镜检查。各种类型软骨肉瘤的肿瘤细胞与非肿瘤性软骨细胞有许多共同特征，例如发达的粗面内质网和微绒毛状细胞质突起。在透明细胞软骨肉瘤中，肿瘤细胞内糖原积累是一个突出特征。间叶性软骨肉瘤的细胞通常表现出未成熟间叶细胞的特征。相比之下，脊索瘤通常含有胞质内有两种类型空泡的泡状细胞。第一种类型最恰当地描述为细胞间物质的胞质内假包涵体，而另一种类型，含糖原的、单膜包被的空泡很可能对应于自噬体（溶酶体）。在所谓的脊索样肉瘤中仅记录到第一种类型的空泡。它们也见于软骨肉瘤中。相比之下，在上述胫骨肿瘤中鉴定出了两种类型的空泡，此外，该肿瘤还表现出脊索瘤的其他细胞学特征。此处呈现的研究结果表明了脊索样肉瘤与软骨源性肿瘤之间存在明显的结构关系。另一方面，我们对这例罕见的胫骨肿瘤的观察表明，与脊索瘤相同的肿瘤可能发生在轴骨以外的部位。
