Ishida T, Oda H, Oka T, Imamura T, Machinami R
Department of Pathology, Faculty of Medicine, University of Tokyo, Japan.
Virchows Arch A Pathol Anat Histopathol. 1993;422(3):239-45. doi: 10.1007/BF01621808.
A case of parachordoma of the left calf in a 19-year-old Chinese female is reported. The tumour showed multinodular growth pattern and consisted of round or oval tumour cells with abundant eosinophilic cytoplasm and myxoid matrix. Tumour cells formed small nests and sometimes showed concentric arrangement. Physaliferous-like cells and undifferentiated spindle cells were occasionally observed among the cell nests. The myxoid matrix was positive for high-iron diamine stain, indicating the presence of chondroitin 4- and 6-sulphates and keratan sulphate. Ultrastructurally, well-developed rough endoplasmic reticulum, abundant intermediate filaments, microvillous cytoplasmic processes, pinocytic vesicles, and desmosome-like junctional structures were found. Tumour cells were positive for S-100 protein and vimentin, but negative for cytokeratin, epithelial membrane antigen, carcinoembryonic antigen, and desmin. These results are consistent with the definition of parachordoma as a soft tissue neoplasm consisting of cells with histology and ultrastructure similar to those of chordoma cells but with immunohistochemistry similar to that of chondroid tumour cells.
报告了1例19岁中国女性左小腿副脊索瘤病例。肿瘤呈多结节生长模式，由圆形或椭圆形肿瘤细胞组成，胞质丰富嗜酸性，伴有黏液样基质。肿瘤细胞形成小巢状，有时呈同心圆排列。在细胞巢中偶尔可见含液样细胞和未分化的梭形细胞。黏液样基质高铁二胺染色呈阳性，表明存在硫酸软骨素4和6以及硫酸角质素。超微结构显示，有发育良好的粗面内质网、丰富的中间丝、微绒毛状胞质突起、吞饮小泡和桥粒样连接结构。肿瘤细胞S-100蛋白和波形蛋白呈阳性，但细胞角蛋白、上皮膜抗原、癌胚抗原和结蛋白呈阴性。这些结果符合副脊索瘤的定义，即一种软组织肿瘤，其细胞的组织学和超微结构与脊索瘤细胞相似，但免疫组化与软骨样肿瘤细胞相似。
