基于CuNi/Fe层状双氢氧化物纳米酶类辣根过氧化物酶活性增强的多巴胺和酪氨酸比色传感
Sensitive colorimetric sensing of dopamine and TYR based on enhanced HRP-like activity of CuNi/Fe LDHs nanozymes.
作者信息
Geng Fenghua, Huang Min, Zhang Xiaoli, Wang Yongxiang, Shao Congying, Xu Maotian
机构信息
Henan Key Laboratory of Biomolecular Recognition and Sensing, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000, China.
Key Laboratory of Green and Precise Synthetic Chemistry and Applications, Ministry of Education, College of Chemistry and Material Science, Huaibei Normal University, Huaibei, 235000, China.
出版信息
Mikrochim Acta. 2025 Mar 1;192(3):197. doi: 10.1007/s00604-025-07056-6.
CuNi/Fe LDHs with HRP-like activity (nanozyme) have been prepared. Contrary to the expected design, free dopamine (DA) was found to greatly enhance the catalytic performances of CuNi/Fe LDHs nanozyme. As far as we know, this is the first report that free DA boosts the catalytic performances of LDHs. Given the superior HRP-like enzyme activity of DA-CuNi/Fe LDHs, a colorimetric method for DA and tyrosinase (TYR) assay with high sensitivity and specificity was established, and it was successfully applied to quantify DA in artificial cerebrospinal fluid and TYR in newborn calf serum. The acquired insights in DA-CuNi/Fe LDHs will contribute to future rational design of other high-performance nanozymes. In addition, the novel DA and TYR assay pave a way for designing further nanozymes-based colorimetric chemo/biosensors.
已制备出具有类辣根过氧化物酶（HRP）活性的铜镍/铁层状双氢氧化物（纳米酶）。与预期设计相反，发现游离多巴胺（DA）能极大地提高铜镍/铁层状双氢氧化物纳米酶的催化性能。据我们所知，这是首次报道游离多巴胺能提高层状双氢氧化物的催化性能。鉴于多巴胺-铜镍/铁层状双氢氧化物具有优异的类HRP酶活性，建立了一种高灵敏度和特异性的比色法用于检测多巴胺（DA）和酪氨酸酶（TYR），并成功应用于定量人工脑脊液中的DA和新生小牛血清中的TYR。对多巴胺-铜镍/铁层状双氢氧化物的深入了解将有助于未来其他高性能纳米酶的合理设计。此外，这种新型的DA和TYR检测方法为进一步设计基于纳米酶的比色化学/生物传感器铺平了道路。
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