CAS Engineering Laboratory for Nanozyme, Key Laboratory of Biomacromolecules (CAS), CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
ACS Nano. 2024 Jan 30;18(4):2533-2540. doi: 10.1021/acsnano.3c07680. Epub 2024 Jan 12.
Nanozymes, nanomaterials exhibiting enzyme-like activities, have emerged as a prominent interdisciplinary field over the past decade. To date, over 1200 different nanomaterials have been identified as nanozymes, covering four catalytic categories: oxidoreductases, hydrolases, isomerases, and lyases. Catalytic activity and specificity are two pivotal benchmarks for evaluating enzymatic performance. Despite substantial progress being made in quantifying and optimizing the catalytic activity of nanozymes, there is still a lack of in-depth research on the catalytic specificity of nanozymes, preventing the formation of consensual knowledge and impeding a more refined and systematic classification of nanozymes. Recently, debates have emerged regarding whether nanozymes could possess catalytic specificity similar to that of enzymes. This Perspective discusses the specificity of nanozymes by referring to the catalytic specificity of enzymes, highlights the specificity gap between nanozymes and enzymes, and concludes by offering our perspective on future research on the specificity of nanozymes.
纳米酶，即具有类似酶活性的纳米材料，是过去十年中出现的一个重要的交叉学科领域。迄今为止，已经有超过 1200 种不同的纳米材料被鉴定为纳米酶，涵盖了氧化还原酶、水解酶、异构酶和裂合酶这四种催化类别。催化活性和特异性是评估酶性能的两个关键基准。尽管在量化和优化纳米酶的催化活性方面已经取得了很大的进展，但对于纳米酶的催化特异性的研究仍然不足，这阻碍了共识知识的形成，并阻碍了对纳米酶更精细和系统的分类。最近，关于纳米酶是否具有类似于酶的催化特异性的争论已经出现。本文通过参考酶的催化特异性来讨论纳米酶的特异性，强调了纳米酶和酶之间的特异性差距，并对未来纳米酶特异性的研究提出了看法。
