Ho C K, Robertson A F, Karp W B
Acta Paediatr Scand. 1985 May;74(3):372-7. doi: 10.1111/j.1651-2227.1985.tb10986.x.
In jaundiced newborn infants, hemolytic disease is considered a risk factor for kernicterus due to the suspected competition between bilirubin and other hemoglobin breakdown products for albumin binding. We have studied the effect of hematin on bilirubin-albumin binding using the peroxidase assay and a light-scattering technique for measuring unbound bilirubin. Our results show that hematin does not affect bilirubin-albumin binding. To determine if other albumin binding functions are affected by hematin, we used a microdialysis rate technique employing two ligands, diazepam and monoacetyldiaminodiphenyl sulfone (MADDS). Hematin does not utilize the diazepam binding function of albumin, but does decrease the albumin binding of MADDS. The results of this study indicate that the MADDS and bilirubin binding functions are not identical. The clinical usefulness of reserve albumin equivalent determination using MADDS is discussed.
在黄疸新生儿中，由于怀疑胆红素与其他血红蛋白分解产物竞争白蛋白结合位点，溶血性疾病被认为是核黄疸的一个危险因素。我们使用过氧化物酶测定法和一种用于测量未结合胆红素的光散射技术，研究了血红素对胆红素 - 白蛋白结合的影响。我们的结果表明，血红素不影响胆红素 - 白蛋白结合。为了确定血红素是否会影响白蛋白的其他结合功能，我们使用了一种微透析速率技术，该技术采用两种配体，即地西泮和单乙酰二氨基二苯砜（MADDS）。血红素不利用白蛋白的地西泮结合功能，但确实会降低白蛋白对MADDS的结合能力。这项研究的结果表明，MADDS和胆红素的结合功能并不相同。文中还讨论了使用MADDS测定储备白蛋白当量的临床实用性。
