Age-related differences in the response of hepatic oxygen consumption to thermal stress in the male garden lizard.

The main aim of the present study is to compare the thermal adaptation capacity of young, middle-aged and old lizards with respect to hepatic oxygen consumption. Cold exposure (1 h at 0-4 degrees C) caused a decline in endogenous oxygen consumption in young but did not evoke significant changes in middle-aged and old lizards. On the other hand, with 5 mM succinate the rate of respiration increased marginally only in cold-exposed old lizards. This suggests that the metabolic response to cold is age-dependent in these lizards. Exposure of homogenates at 40 +/- 1 degree C for 15 min led to a rise in the hepatic oxygen consumption of young but not in that of either middle-aged or old lizards. There were no changes in the rate of oxygen consumption upon exposure for a similar period at 45 +/- 1 degree C of liver homogenates from lizards of three different age groups. However, exposure at 51 +/- 1 degree C resulted in a higher degree of decline in the rate of hepatic oxygen consumption in middle-aged and old than in young lizards. Thus, the young lizards appear to be better-adapted to hyperthermic stress than the middle-aged and old.