Soozangar Narges, Jeddi Farhad, Spotin Adel, Molaei Soheila, Mohammadi-Ghalehbin Behnam, Habibzadeh Shahram, Mohammadshahi Jafar, Mirzanejad-Asl Hafez, Dogaheh Hadi Peeri
Zoonoses Research Center, Ardabil University of Medical Sciences, Ardabil, Iran.
Department of Genetics and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
BMC Infect Dis. 2025 Mar 5;25(1):316. doi: 10.1186/s12879-025-10728-6.
Crimean-Congo hemorrhagic fever (CCHF) is one of the most acute tick-transmitted zoonotic diseases. The purpose of this study was to determine the molecular characteristics and phylogenetic analysis of the CCHF virus based on the S-segment nucleocapsid gene in Ardabil Province, northwestern Iran.
From November 2021 to May 2023, a total of 20 peripheral blood specimens were collected from suspected CCHF patients. Following RNA extraction, the partial nucleotide sequence of the S fragment was amplified (536 bp) using a one-step RT-PCR Kit. PCR products were successfully sequenced to perform phylogenetic analysis and haplotype distribution.
The mean age of CCHF patients was 30.4 ± 10.45 years and all male patients had a history of tick exposure. According to molecular findings, the frequency of CCHFV in patients was 25% (5/20). A high haplotype diversity (Hd: 1; haplotype number: 5) and distinct genetic clade (IV) of CCHFV were identified among the patients in Ardabil province compared to other regions of Iran.
The occurrence of new haplotypes shows new evidence of the emerging threat of the CCHFV in the region. Current findings strengthen our knowledge of transmission dynamics and dispersion of probable drug-resistant alleles of CCHFV in northwestern Iran, also it will become the basis of public health policy to control CCHF in the region.
Not applicable.
克里米亚-刚果出血热（CCHF）是最严重的蜱传人畜共患病之一。本研究旨在基于伊朗西北部阿尔达比勒省CCHF病毒的S片段核衣壳基因，确定其分子特征并进行系统发育分析。
2021年11月至2023年5月，共收集了20份疑似CCHF患者的外周血标本。提取RNA后，使用一步法RT-PCR试剂盒扩增S片段的部分核苷酸序列（536 bp）。对PCR产物成功测序以进行系统发育分析和单倍型分布研究。
CCHF患者的平均年龄为30.4±10.45岁，所有男性患者均有蜱叮咬史。根据分子检测结果，患者中CCHFV的检出率为25%（5/20）。与伊朗其他地区相比，在阿尔达比勒省的患者中发现了CCHFV的高单倍型多样性（Hd：1；单倍型数量：5）和独特的遗传分支（IV）。
新单倍型的出现表明该地区CCHFV出现新威胁的新证据。当前研究结果加强了我们对伊朗西北部CCHFV传播动态和可能的耐药等位基因扩散的认识，也将成为该地区控制CCHF公共卫生政策的基础。
不适用。
