Onorato Irene, Tzanou Athanasia, Schneider Marius, Uran Cem, Broggini Ana Clara, Vinck Martin
Ernst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, 60528 Frankfurt am Main, Germany; Max Planck Institute for Brain Research, 60438 Frankfurt, Germany; Neuroscience Research Center, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.
Ernst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, 60528 Frankfurt am Main, Germany.
Cell Rep. 2025 Mar 25;44(3):115385. doi: 10.1016/j.celrep.2025.115385. Epub 2025 Mar 5.
Gamma-frequency oscillations are a hallmark of active information processing and are generated by interactions between excitatory and inhibitory neurons. To examine the contribution of distinct inhibitory interneurons to visually induced gamma oscillations, we recorded from optogenetically identified PV+ (parvalbumin) and Sst+ (somatostatin) interneurons in mouse primary visual cortex (V1). PV and Sst inhibitory interneurons exhibited distinct correlations to gamma oscillations. PV cells were strongly phase locked, while Sst cells were weakly phase locked, except for narrow-waveform Sst cells. PV cells fired at a substantially earlier phase in the gamma cycle (≈6 ms) than Sst cells. PV cells fired shortly after the onset of tightly synchronized burst events in excitatory cells, while Sst interneurons fired after subsequent burst spikes or single spikes. These findings indicate a main role of PV interneurons in synchronizing network activity and suggest that PV and Sst interneurons control the excitability of somatic and dendritic neural compartments with precise time delays coordinated by gamma oscillations.
伽马频率振荡是活跃信息处理的一个标志，由兴奋性和抑制性神经元之间的相互作用产生。为了研究不同抑制性中间神经元对视觉诱发的伽马振荡的贡献，我们在小鼠初级视觉皮层（V1）中对通过光遗传学鉴定的小清蛋白阳性（PV+）和生长抑素阳性（Sst+）中间神经元进行了记录。PV和Sst抑制性中间神经元与伽马振荡表现出不同的相关性。PV细胞强烈锁相，而Sst细胞弱锁相，但窄波形Sst细胞除外。PV细胞在伽马周期中比Sst细胞早得多的相位（约6毫秒）放电。PV细胞在兴奋性细胞中紧密同步爆发事件开始后不久放电，而Sst中间神经元在随后的爆发尖峰或单个尖峰之后放电。这些发现表明PV中间神经元在同步网络活动中起主要作用，并表明PV和Sst中间神经元通过伽马振荡协调的精确时间延迟来控制体细胞和树突状神经节段的兴奋性。
