Khan Kfait Ullah, Rehman Misbahur, Hameed Mariam, Hayat Khyzar, Saeed Farhan, Afzaal Muhammad, Akram Noor, Ahmed Faiyaz, Asghar Aasma, Hailu Gebremichael Gebremedhin
Department of Chemistry, University of Lahore, Sargodha Campus, Punjab, Pakistan.
Department of Food Science, Government College University Faisalabad, Faisalabad, Pakistan.
PLoS One. 2025 Mar 12;20(3):e0314850. doi: 10.1371/journal.pone.0314850. eCollection 2025.
This study investigates the synthesis and characterization of Plant-Ag-graphene nanocomposites through a combination of spectroscopic and microscopic techniques, the nanocomposites were formed by catalyzing silver nanoparticles with plant extracts, and the resulting structures were analyzed using advanced instrumentation. In the FTIR analysis, distinctive peaks were observed at 3340 cm⁻1 (O-H stretching), 1740 cm⁻1 (C = O stretching), and 1050 cm⁻1. When compared to silver nanoparticles, the nanocomposites exhibited altered peak intensities, indicating modifications in chemical bonding. SEM images revealed that in nanocomposites, nanoparticles were adhered to graphene sheets, confirming the successful formation of Plant-Ag-graphene structures. EDX spectra showed a reduction in the silver content, confirming the integration of graphene into the nanocomposites. XRD analysis confirmed the presence of face-centered cubic-shaped Ag metal in the nanocomposites, while graphene exhibited a hexagonal crystalline shape. UV-Vis spectroscopy demonstrated shifts in peak positions, Spectrum A (400 to 700 nm) and spectrum B (265 nm), suggesting the successful synthesis of Plant-Ag-graphene nanocomposites. Moreover, the cytotoxic activity showed cell inhibition among Plant-Ag-Graphene (65.69%) and Plant-Ag (61.39%), respectively.
本研究通过光谱和显微镜技术相结合的方法，对植物-银-石墨烯纳米复合材料的合成与表征进行了研究。纳米复合材料是通过用植物提取物催化银纳米颗粒形成的，并使用先进仪器对所得结构进行了分析。在傅里叶变换红外光谱（FTIR）分析中，在3340 cm⁻¹（O-H伸缩振动）、1740 cm⁻¹（C=O伸缩振动）和1050 cm⁻¹处观察到明显的峰。与银纳米颗粒相比，纳米复合材料的峰强度发生了变化，表明化学键合发生了改变。扫描电子显微镜（SEM）图像显示，在纳米复合材料中，纳米颗粒附着在石墨烯片上，证实了植物-银-石墨烯结构的成功形成。能量色散X射线光谱（EDX）显示银含量降低，证实了石墨烯已整合到纳米复合材料中。X射线衍射（XRD）分析证实了纳米复合材料中存在面心立方形状的银金属，而石墨烯呈现六边形晶体形状。紫外可见光谱显示峰位发生了移动，光谱A（400至700 nm）和光谱B（265 nm），表明植物-银-石墨烯纳米复合材料的成功合成。此外，细胞毒性活性分别显示植物-银-石墨烯（65.69%）和植物-银（61.39%）对细胞有抑制作用。
