Lopes Pedro Henrique Ribeiro, Pereira Nicaely Maria de Oliveira, da Rocha Matheus Nunes, Marinho Marcia Machado, Guedes Jesyka Macêdo, Rodrigues Tigressa Helena Soares, Do Vale Jean Parcelli Costa, Marinho Emmanuel Silva, Santiago Gilvandete Maria Pinheiro, Santos Hélcio Silva Dos
Postgraduate Program in Natural Sciences, Ceará State University, Fortaleza 60714-903, CE, Brazil.
Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza 60020-181, CE, Brazil.
Molecules. 2025 Feb 24;30(5):1034. doi: 10.3390/molecules30051034.
The mosquito is the primary vector of dengue, a neglected disease and a serious public health problem in tropical countries. The control of this vector has been carried out using chemical insecticides, which impact human health. Thus, it is essential to develop natural larvicides that are less harmful to the environment. This study investigates the circadian cycle and larvicidal activity of essential oils from against . The leaf oils were extracted by hydrodistillation and analyzed by GC-MS and GC-FID. The circadian study revealed variations in the chemical composition of oils extracted at different times of the day. The main constituents were , , , , , and . The larvicidal activity showed LC values at the following different collection times: 55.294 ± 3.209 μg/mL at 08:00 h; 95.485 ± 2.684 μg/mL at 12:00 h; and 64.883 ± 1.780 μg/mL at 17:00 h. Molecular docking simulations indicated that , , , and strongly interact with the active site of the sterol carrier protein, suggesting their role in larvicidal activity. These findings reinforce the potential of essential oils as an alternative for control. The predictive pharmacokinetic tests showed a PAMPA profile associated with high effective cellular permeability and microsomal stability, resulting from the metabolic stability of the derivatives (3) and (6) , indicating that these compounds have the highest pharmacokinetic viability and low reactivity with respect to organ toxicity.
蚊子是登革热的主要传播媒介，登革热是一种被忽视的疾病，在热带国家是严重的公共卫生问题。人们一直使用化学杀虫剂来控制这种传播媒介，而化学杀虫剂会对人类健康产生影响。因此，开发对环境危害较小的天然杀幼虫剂至关重要。本研究调查了[植物名称]精油对[蚊虫名称]的昼夜节律周期和杀幼虫活性。通过水蒸馏法提取叶油，并采用气相色谱-质谱联用仪（GC-MS）和气相色谱-火焰离子化检测器（GC-FID）进行分析。昼夜节律研究揭示了在一天不同时间提取的油的化学成分存在差异。主要成分有[成分名称1]、[成分名称2]、[成分名称3]、[成分名称4]、[成分名称5]和[成分名称6]。杀幼虫活性显示在以下不同采集时间的半数致死浓度（LC）值：08:00时为55.294±3.209μg/mL；12:00时为95.485±2.684μg/mL；17:00时为64.883±1.780μg/mL。分子对接模拟表明，[成分名称1]、[成分名称2]、[成分名称3]和[成分名称4]与甾醇载体蛋白的活性位点强烈相互作用，表明它们在杀幼虫活性中发挥作用。这些发现增强了[植物名称]精油作为[蚊虫名称]控制替代物的潜力。预测性药代动力学测试显示，由于衍生物（3）和（6）的代谢稳定性，其平行人工膜渗透试验（PAMPA）谱与高有效细胞通透性和微粒体稳定性相关，表明这些化合物具有最高的药代动力学活力，且对器官毒性的反应性较低。
