Jin Ting, Wu Chunhua, Huang Zhen, Zhang Xingguo, Li Shimeng, Ding Chao, Long Weihua
College of Rural Revitalization, Jiangsu Open University, Nanjing 210036, China.
College of Agronomy, Nanjing Agricultural University, Nanjing 211800, China.
Int J Mol Sci. 2025 Mar 6;26(5):2373. doi: 10.3390/ijms26052373.
The Aldehyde Dehydrogenase (ALDH) superfamily comprises a group of NAD or NADP-dependent enzymes that play essential roles in responding to abiotic stresses in plants. In L., however, the increasing frequency of extremely low temperatures during winter in recent years has significantly affected both yield and quality. This study conducted a genome-wide screening of superfamily genes, analyzing their gene structures, evolutionary relationships, protein physicochemical properties, and expression patterns under low-temperature stress to explore the function of the superfamily gene in cold tolerance in L. A total of six genes with significant differences in expression levels were verified utilizing quantitative real-time polymerase chain reaction (qRT-PCR), revealing that , , , , , and all exhibited higher expression in cold-tolerant material 24W233 compared with cold-sensitive material 24W259. Additionally, a single nucleotide polymorphism (SNP) in the promoter region shows differences between the cold-tolerant (24W233) and the cold-sensitive (24W259) varieties, and it may be associated with the cold tolerance of these two varieties. This comprehensive analysis offers valuable insights into the role of family genes in low-temperature stress adaptation in and offers genetic resources for the development of novel cold-tolerant cultivars.
醛脱氢酶（ALDH）超家族由一组NAD或NADP依赖性酶组成，这些酶在植物应对非生物胁迫中发挥着重要作用。然而，近年来，在L.中，冬季极端低温出现的频率增加，显著影响了产量和品质。本研究对超家族基因进行了全基因组筛选，分析了它们的基因结构、进化关系、蛋白质理化性质以及低温胁迫下的表达模式，以探究超家族基因在L.耐寒性中的功能。利用实时定量聚合酶链反应（qRT-PCR）验证了总共六个表达水平有显著差异的基因，结果表明，与冷敏感材料24W259相比，、、、、和在耐寒材料24W233中均表现出更高的表达。此外，启动子区域的一个单核苷酸多态性（SNP）在耐寒（24W233）和冷敏感（24W259）品种之间存在差异，并且它可能与这两个品种的耐寒性有关。这一综合分析为超家族基因在L.低温胁迫适应中的作用提供了有价值的见解，并为新型耐寒品种的培育提供了遗传资源。
