Cordera R, Andraghetti G, Bonadonna R, Gherzi R, Maiello M, Odetti P, Viviani G, Berri F, Adezati L
Horm Metab Res. 1985 Apr;17(4):201-4. doi: 10.1055/s-2007-1013491.
In vitro the rate of synthesis of the aldiminic linkage between Hb and glucose depends on glucose concentration, length of incubation and some other physiological factors. To understand better the regulation of this synthesis and to verify the role of cell age and of basal HbA1 levels on the rate of synthesis of pre-A1, we studied red cells from 7 normal controls and 7 diabetics, with high HbA1 levels. We found that the content of HbA1 (stable glycosylated hemoglobin) is able to negatively affect the rate of synthesis of new pre-A1, according to a curvilinear model. These results suggest that in vitro the glycosylation process is saturable, and that elevated values of HbA1 are able to slow the synthesis of pre-A1 in vitro.
在体外，血红蛋白（Hb）与葡萄糖之间醛亚胺键的合成速率取决于葡萄糖浓度、孵育时间以及其他一些生理因素。为了更好地理解这种合成的调节机制，并验证细胞年龄和基础糖化血红蛋白A1（HbA1）水平对前A1合成速率的作用，我们研究了7名正常对照者和7名HbA1水平较高的糖尿病患者的红细胞。我们发现，根据曲线模型，HbA1（稳定的糖化血红蛋白）的含量能够对新的前A1的合成速率产生负面影响。这些结果表明，在体外糖基化过程是可饱和的，并且升高的HbA1值能够减缓体外前A1的合成。
