State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China.
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Genes (Basel). 2020 Sep 27;11(10):1134. doi: 10.3390/genes11101134.
The 12-oxo-phytodienoic acid reductases () have been proven to play a major role in plant development and growth. Although the classification and functions of have been well understood in Arabidopsis, tomato, rice, maize, and wheat, the information of genes in cotton genome and their responses to biotic and abiotic stresses have not been reported. In this study, we found 10 and 9 genes in and , respectively. They were classified into three groups, based on the similar gene structure and conserved protein motifs. These genes just located on chromosome 01, chromosome 05, and chromosome 06. In addition, the whole genome duplication (WGD) or segmental duplication events contributed to the evolution of the gene family. The analyses of cis-acting regulatory elements of showed that the functions of genes in cotton might be related to growth, development, hormone, and stresses. Expression patterns showed that were upregulated under salt treatment and repressed by polyethylene glycol 6000 (PEG6000). The expression patterns of were different in leaf, root, and stem under infection. showed a higher expression level than other genes in cotton root. The virus-induced gene silencing (VIGS) analysis suggested that knockdown of could increase the susceptibility of cotton to infection. Furthermore, also modulated the expressions of jasmonic acid (JA) pathway-regulated genes under the infection. Overall, our results provided the evolution and potential functions of the genes in cotton. These findings suggested that might play an important role in cotton resistance to .
12-氧代-植物二烯酸还原酶（）已被证明在植物发育和生长中起主要作用。尽管在拟南芥、番茄、水稻、玉米和小麦中对的分类和功能已有很好的了解，但棉花基因组中基因的信息及其对生物和非生物胁迫的反应尚未报道。在这项研究中，我们在和中分别发现了 10 个和 9 个基因。它们根据相似的基因结构和保守的蛋白质基序分为三组。这些基因仅位于染色体 01、染色体 05 和染色体 06 上。此外，全基因组复制（WGD）或片段复制事件促成了基因家族的进化。对基因的顺式作用调控元件的分析表明，棉花基因的功能可能与生长、发育、激素和胁迫有关。表达模式表明，在盐处理下上调，而聚乙二醇 6000（PEG6000）抑制表达。在叶、根和茎中，的表达模式在感染后不同。在棉花根中比其他基因表达水平更高。病毒诱导的基因沉默（VIGS）分析表明，基因的敲低可增加棉花对感染的敏感性。此外，在感染下，还调节了茉莉酸（JA）途径调控基因的表达。总体而言，我们的研究结果提供了棉花中基因的进化和潜在功能。这些发现表明，在棉花对的抗性中可能发挥重要作用。
