通过交叉放射免疫电泳鉴定甲壳类过敏原。

Identification of crustacea allergens by crossed radioimmunoelectrophoresis.

作者信息

Lehrer S B, McCants M L, Salvaggio J E

出版信息

Int Arch Allergy Appl Immunol. 1985;77(1-2):192-4. doi: 10.1159/000233783.

Abstract

Crossed immunoelectrophoresis (CIE) detected 18 precipitating antigens in extracts of shrimp. Of these antigens, crossed-line immunoelectrophoresis (CLIE) of shrimp extract demonstrated that 5 cross-reacted with crayfish, 3 with lobster and 1 with crab extract. Allergens present in the shrimp CIE plates were identified by crossed radioimmunoelectrophoresis (CRIE) using sera from 6 study subjects who were skin-test and RAST positive to shrimp extract. Of the 7 allergens detected, 3 (precipitins 1, 3 and 6) reacted with most of the 6 sera tested from shrimp-sensitive subjects. Precipitins 1 and 6 appear to be common crustacea allergens (present in shrimp, crayfish, lobster and crab) whereas precipitin 3 may be a specific allergen since it is present only in shrimp.

摘要

交叉免疫电泳（CIE）检测到虾提取物中有18种沉淀抗原。在这些抗原中，虾提取物的交叉线免疫电泳（CLIE）表明，其中5种与小龙虾提取物交叉反应，3种与龙虾提取物交叉反应，1种与蟹提取物交叉反应。使用6名对虾提取物皮肤试验和放射性变应原吸附试验（RAST）呈阳性的研究对象的血清，通过交叉放射免疫电泳（CRIE）鉴定虾CIE板中存在的过敏原。在检测到的7种过敏原中，3种（沉淀素1、3和6）与6名对虾敏感的研究对象的大多数血清发生反应。沉淀素1和6似乎是常见的甲壳类过敏原（存在于虾、小龙虾、龙虾和蟹中），而沉淀素3可能是一种特异性过敏原，因为它仅存在于虾中。